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Preparation Of Protein-Steroid Conjugates And Antisera Against 0Estradi0L-17β

Framstälining au protein-steroid konjugat och antisera mot 17β-estradiol

Acta Veterinaria Scandinavica volume 14, pages 278–291 (1973)Cite this article

Abstract

A conjugate of oestradiol-17β and bovine serum albumin (BSA) has been prepared by the mixed anhydride method. The conjugate was characterized by u.v.-analyses and immunoelectrophoresis, and the number of moles of oestradiol-17β per mole of BSA was found to be 25. The conjugate was used for immunization of two sheep and one rabbit in order to elict antisera against oestradiol-17β. Antibodies could be detected in all three animals after 5–16 weeks depending on the route of immunization. The antiserum from one sheep could be used in a radioimmunoassay system for oestradiol-17β in a dilution of 1:5000 and was found to cross-react with oestrone and oestradiol-17α to a lesser extent than another antiserum against oestradiol-17β.

Sammanfattning

Ett konjugat av 17β-estradiol och bovint serum albumin (BSA) har framställts med „mixed anhydride“ metoden. Konjugatet karaktariserades med u.v.-analys och immunoelektrofores och antalet mol av 17β-estradiol per mol BSA beräknades till 25. Konjugatet användes for immunisering av två får och en kanin for att framställa antisera mot 17β-estradiol. Antikroppar kunde pävisas hos alla tre djuren efter 5–16 veckor. Antiserum från ett får kunde användas i ett radioimmunologiskt system för 17β-estradiol i en spädning 1:5000 och korsreagerande med estrone och 17α-estradiol i mindre utsträckning i jämförelse med ett annat antiserum mot 17β-estradiol.

References

  • Abraham, G. E.: Solid-phase radioimmunoassay of estradiol-17. J. clin. Endocr. 1969, 29, 866–870.

    Article  CAS  Google Scholar 

  • Erlanger, B. F., F. Borek, S. Beiser & S. Liebermann: Preparation and characterization of conjugates of bovine serum albumin with testosteron and with cortisone. J. biol. Chem. 1958, 228, 713–727.

    Article  Google Scholar 

  • Erlanger, B. F., S.M. Beiser, F. Borek, F. Edel & S. Liebermann: In Williams, C. A. and M. C. Chase, eds. Methods in Immunology and Immunochemistry. Acad. Press Inc. Vol. I, pp. 144, 151, New York 1967.

  • Ferin, M., P. E. Zimmering, S. Liebermann & R. L. Vande Wiele: Inactivation of the biological effects of exogenous and endogenous estrogens by antibodies to 17β-estradiol. Endocrinology 1968, 83, 565–571.

    Article  CAS  Google Scholar 

  • Goodfriend, L. & A. H. Sehon: Antibodies to estrone-protein conjugates. I. Immunochemical studies. Ganad. J. Biochem. 1961, 39, 941–960.

    CAS  Google Scholar 

  • Gross, S. J., D. H. Campbell & H. H. Weetall: Production of antisera to steroids coupled to proteins directly through the phenolic A ring. Immunochemistry 1968, 5, 55–65.

    Article  CAS  Google Scholar 

  • Hotchkiss, J., L. E. Atkinson & E. Knibil: Time course of serum estrogen and luteinizing hormone (LH) concentrations during the menstrual cycle of the Rhesus monkey. Endocrinology 1971, 89, 177–183.

    Article  CAS  Google Scholar 

  • Landsteiner, K.: Specificity of Serological Reactions. Harward Univ. Press, Cambridge, Mass. 1946.

  • Liebermann, S., B. F. Erlanger, S. M. Beiser & F. J. Agate: Steroid-protein conjugates: Their chemical, immunochemical and endocrinological properties. Recent Progr. Hormone Res. 1959, 15, 165–196.

    Google Scholar 

  • Peron, F. G. & B. V. Caldwell: Immunological methods in steroid determination. Appleton-Century-Crofts. Education Division, Meredith Corporation, New York 1970.

  • Scheideggar, J. J. & J. Zalund: Étude immunoélectrophorétique et électrophorétique des protéines sériques dans certaines affections hépatiques. (Immunoelectrophoresis and electrophoresis of serum proteins of liver diseases). Helv. med. Acta 1957, 23, 499–504.

    Google Scholar 

  • Yalow, R. S. & S. A. Berson: Immunoassay of endogenous plasma insulin. J. clin. Invest. 1960, 39, 1157–1175.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Medicine I, Royal Veterinary College, S-104 05, Stockholm 50, Sweden

    Kjell Martinsson, Paul Lindberg & Elof D. B. Johansson

  2. Department of Obstetrics and Gynaecology, University of Uppsala, Uppsala, Sweden

    Kjell Martinsson, Paul Lindberg & Elof D. B. Johansson

Authors
  1. Kjell Martinsson
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  2. Paul Lindberg
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  3. Elof D. B. Johansson
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Additional information

This study was supported by the Swedish Medical Research Council (B 72-17x-3495-01), the Ford Foundation, the Population Council, and the John M. Olin Foundation.

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Martinsson, K., Lindberg, P. & Johansson, E.D.B. Preparation Of Protein-Steroid Conjugates And Antisera Against 0Estradi0L-17β. Acta Vet Scand 14, 278–291 (1973). https://doi.org/10.1186/BF03547446

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Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147