- Published:
Indirect Fluorescent Antibody Techniques for Demonstration of Trout Viruses and Corresponding Antibody
Indirekte immunfluorescens-teknik til påvisning af ørredvirus og ørredantistof
Acta Veterinaria Scandinavica volume 15, pages 198–205 (1974)
Abstract
Two variations of the indirect fluorescent antibody technique (FAT) have been utilized in the work concerning two important virus diseases of trout, viral haemorrhagic septicaemia (VHS) and infectious pancreatic necrosis (IPN). A “two layer” indirect FAT allowed demonstration of the respective viruses in cell cultures and a “three layer” indirect FAT allowed demonstration of trout antibody to the viruses. Antibody, by means of the latter technique, could be demonstrated only in artificially immunized trout.
Sammendrag
To ørredpatogene virus (Egtved og IPN virus) er påvist i inficerede cellekulturer ved hjælp af en „to-lags“ indirekte immunfluorescensteknik. Ved hjælp af en „tre-lags“ indirekte immunfluorescensteknik kunne antistof overfor de pågældende virus påvises tidligere end eller samtidig med neutraliserende antistof i serum fra kunstigt immuniserede ørreder. Metoden tillod ikke påvisning af antistof i ørreder udsat for naturlig smitte med Egtved eller IPN virus.
References
Goldman, M.: Fluorescent Antibody Methods. Academic Press, New York and London 1968, p. 149.
Grauballe, P. C: Infektiøs Pankreasnekrose. En ny sygdom i Danmark hos laksefisk. Diagnostiske og aetiologiske undersøgelser. (Infectious pancreatic necrosis. A new disease of salmonids in Denmark. Diagnostic and aetiological investigations). Licentiatafhandling. Den Kgl. Veterinær- og Landbohøjskole, København 1972, pp. 1–63.
Gravell, M. & R. G. Malsberger: A permanent cell line from the fathead minnow (Pimephales promelas). Ann. N.Y. Acad. Sci. 1965, 126, 555–565.
Jensen, M. H.: Research on the virus of Egtved disease. Ann. N.Y. Acad. Sci. 1965, 126, 422–426.
Jørgensen, P. E. Vestergård: Serological identification of Egtved virus. Bull. Off. int. Epiz. 1969, 69, 985–989.
Jørgensen, P. E. Vestergård: Egtved virus: Demonstration of neutralizing antibodies in artificially infected rainbow trout (Salmo gairdneri). J. Fish Res. Bd. Ganada 1971, 28, 875–877.
Jørgensen, P. E. Vestergård: Freund’s adjuvants: Their influence on the specificity of viral antisera. Acta path, microbiol. scand. Section B. 1972 a, 80, 931.
Jørgensen, P. E. Vestergård: Egtved virus: Antigenic variation in 76 virus isolates examined in neutralization tests and by means of the fluorescent antibody technique. In “Symp. zool. Soc. London No. 30”. L. Mawdsley-Thomas, ed. Academic Press, London and New York 1972 b, 333–340.
Jørgensen, P. E. Vestergård: The nature and biological activity of IPN virus neutralizing antibodies in normal and immunized rainbow trout. Arch. ges. Virusforsch. 1973, 42, 9–20.
Jørgensen, P. E. Vestergård & A. Meyling: Egtved virus: Demonstration of virus antigen by the fluorescent antibody technique in tissues of rainbow trout affected by viral haemorrhagic septicaemia and in cell cultures infected with Egtved virus. Arch. ges. Virusforsch. 1972, 36, 115–122.
Wolf, K. & M. C. Quimby: Established eurythermic line of fish cells in vitro. Science 1962, 135, 1065–1066.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vestergárd Jørgensen, P.E. Indirect Fluorescent Antibody Techniques for Demonstration of Trout Viruses and Corresponding Antibody. Acta Vet Scand 15, 198–205 (1974). https://doi.org/10.1186/BF03547481
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1186/BF03547481