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Diurnal Variation of Peripheral Plasma Levels of Testosterone in Bulls Measured by a Rapid Radioimmunoassay Procedure

Dygnsvariationen av blodplasmanivåer av testosteron hos tjur mätt med en radioimmunologisk metod

Acta Veterinaria Scandinavica volume 15, pages 90–99 (1974)Cite this article

Abstract

A radioimmunoassay technique, using antibodies to testosterone-3-(O-carboxymethyl) oxime-bovine serum albumin, has been applied for the measurement of peripheral plasma levels of testosterone in bulls. The individual as well as the mean 24-hr. pattern of peripheral plasma levels of testosterone in the four bulls investigated showed three distinct peaks. The mean peripheral plasma level of testosterone peaked around 10 p.m., around 6 a.m. and around noon, the mean levels obtained at these times being 6.2, 6.1 and 4.2 ng/ml, respectively. In one bull an additional peak occurred around 2 a.m. (8.2 ng/ml). Significantly lower peripheral plasma levels of testosterone were observed between the peak values.

Sammanfattning

En radioimmunologisk metod, utnyttjande antikroppar mot testosteron-3-(O-carboyxmetyl)oxim-bovint serum albumin, har applicerats för bestämning av plasma nivåer av testosteron hos tjur. Hos samtliga fyra tjurar, som undersöktes med provtagningar under 24 timmar, uppvisade plasmanivåerna av testosteron ett gemensamt mönster med tre distinkta koncentrationsökningar. Medelplasmanivån av testosteron var högst omkring kl. 22, omkring kl. 6 och omkring kl. 12. Medelnivåerna vid dessa tillfällen var respektive 6,2, 6,1 och 4,2 ng/ ml. En tjur uppvisade även en koncentrationsökning omkring kl. 14 (8,2 ng/ml). Signifikant lägre plasma nivåer av testosteron uppmättes på tidpunkter melian de höga testosteronvärdena.

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Author notes

  1. Prakash Chandra Sanwal

    Present address: Department of Physiology and Endocrinology, Indian Veterinary Research Institute, Izatnagar, U.P., India

Authors and Affiliations

  1. Department of Clinical Biochemistry, Royal Veterinary College, Stockholm, Sweden, and the Department of Physiology, Veterinary College of Norway, Oslo, Norway

    Prakash Chandra Sanwal, Anne Sundby & Lars-Eric Edqvist

Authors
  1. Prakash Chandra Sanwal
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  2. Anne Sundby
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  3. Lars-Eric Edqvist
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Additional information

This study was supported by the Agricultural Research Council of Norway and the Swedish Council for Forestry and Agricultural Research

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Sanwal, P.C., Sundby, A. & Edqvist, LE. Diurnal Variation of Peripheral Plasma Levels of Testosterone in Bulls Measured by a Rapid Radioimmunoassay Procedure. Acta Vet Scand 15, 90–99 (1974). https://doi.org/10.1186/BF03547496

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Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147