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Antigen-Antibody Crossed Electro-Phoretic Studies and Quantitative Comparisons of Serum Transferrin Types in Horses

Undersøkeiser ved antigen-antistoff krysset elektroforese og kvantitative sammenligninger av transferrin-typer i serum hos hester

Abstract

Selected transferrin phenotypes from 14 horses were investigated by antigen-antibody crossed electrophoresis. Horse sera were subjected to starch gel electrophoresis followed by right angle electrophoresis in agarose gels containing rabbit produced anti-horse transferrin. This technique gave an additional zone in the front as compared with 2 transferrin zones seen after ordinary starch gel electrophoresis.

Comparisons of transferrin concentrations in horse sera were performed by an immunodiffusion technique. Values were related to a chosen reference serum. A total of 372 horses (210 Norwegian Trotters and 162 Warmblood Trotters) were investigated. There were considerable variations among horses, with the highest transferrin value 196% and the lowest 55% of the standard.

There were highly significant differences between average values of certain transferrin phenotypes, the highest being TfFF and the lowest TfDO, TfFO and TfRR. In animals with the same transferrin phenotype, the average transferrin values were significantly higher in foals than in mares.

No difference in transferrin values were found between sexes.

Sammendrag

Utvalgte transferrin-typer fra 14 hester ble undersøkt ved antigen-antistoff-elektroforese. Hesteserum ble undersøkt ved hjelp av stivelsesgel-elektroforese etterfulgt av elektroforese på agarose gel som inneholdt antihest-transferrin fremstillet på kanin. Denne teknikk ga en ekstra sone i fronten sammenlignet med to transferrinsoner som sees etter vanlig stivelsesgel-elektroforese.

Sammenligninger av transferrin-konsentrasjoner i hestesera ble utført ved anvendelse av Mancini et al.’s immundiffusjonsteknikk. Relative verdier i forhold til et valgt referanseserum ble bestemt for i alt 372 hester (210 norske travere og 162 varmblods travere). Det var betydelig variasjon mellom hestene, den høyeste transferrinverdi var 196% og den laveste 55%.

Mellom visse transferrin-fenotyper var det høysignifikante forskjeller mellom middelverdier, TfFF var den høyeste og TfDO, TfFO og TfRR de laveste. Hos dyr med samme transferrin-fenotype var middelverdiene signifikant høyere hos føll enn hos hopper.

Det ble ikke funnet noen forskjell på transferrin-verdier mellom kjønnene.

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Ek, N. Antigen-Antibody Crossed Electro-Phoretic Studies and Quantitative Comparisons of Serum Transferrin Types in Horses. Acta Vet Scand 22, 246–259 (1981). https://doi.org/10.1186/BF03547514

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Keywords

  • horse serum
  • transferrin phenotypes
  • relative transferrin quantities