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Adenosine Deaminase and Porcine Meat Quality
I. Effect of Dipyridamole on Plasma Free Fatty Acids, Glucose, Lactate and c-AMP in Pigs Representing High and Low Red Cell Adenosine Deaminase Activity
Adenosin deaminase og kødkvalitet hos svin: Effekt af adenosin på plasma frie fede syrer, glucose, laktat og c-AMP hos grise repræsenterende høj og lav erytrocyt adenosin deaminase aktivitet
Acta Veterinaria Scandinavica volume 31, pages 137–143 (1990)
Abstract
The effect of dipyridamole - an adenosine uptake inhibitor - on the plasma concentration of free fatty acids (FFA), glucose, lactate and cyclic adenosine monophosphate (cAMP) has been examined in 2 groups of Landrace pigs representing low (Ada 0) and high (Ada A) red cell adenosine deaminase (Ada) activity. Pigs fitted with a jugular vein catheter were given dipyridamole (0.16 mg/kg/min) over a period of 30 min. The infusions were performed 22 h after the last meal at a time where pigs were found to show steady increase and decline in rates of lipolysis and glycogenosis, respectively. The results showed that lipid mobilization as identified by the plasma FFA concentration was markedly depressed. During the infusion of dipyridamole similar degree of inhibition was seen in Ada 0 and Ada A pigs, however, in the period following the infusion, a significantly stronger suppression persisted in the Ada 0 pigs. Both the blood glucose and lactate level rose distinctly as a result of the dipyridamole treatment. This stimulation of the glycolysis rate was significantly more expressed in Ada 0 pigs compared to that of the Ada A pigs. When theophylline, an antagonist of adenosine, was given together with dipyridamole, the rise in the lactate level was considerably diminished. Dipyridamole also produced a distinct rise in the plasma cAMP levels.
Sammenfatning
Som led i at forklare den fundne sammenhæng mellem det polymorfe enzymsystem adenosin deaminase (Ada) og kødkvaliteten hos svin er en direkte effekt af erytrocyt Ada aktiviteten og adenosin på skeletmuskelstofskiftet søgt underbygget. Landrace grise repræsenterende høj (Ada A) og lav (Ada 0) erytrocyt Ada aktivitet er via et kateter indlagt i V. jugularis tilført dipyridamol (0,16 mg/kg/min) over en periode på 30 min. Infusionerne blev foretaget på et tidspunkt, hvor dyrene som følge af faste udviste en stigende og faldende hastighed af henholdsvis lipolysen og glykogenolysen. Resultaterne af dipyridamoltilførslen viste, at fedtmobiliseringen udtrykt ved plasmaets FFA indhold blev markant hæmmet. Hæmningen varede signifikant længere hos Ada 0 grisene. Samtidig med faldet i plasma FFA sås en distinkt stigning i såvel glucose- og laktatindholdet. Stigningen i blodets laktatindhold og dermed i glykolysehastigheden var signifikant mere udtalt hos Ada 0 grisene. Ved at tilføre theophyllin - en antagonist til adenosin - sammen med dipyridamol sås en markant mindre stigning i laktatindholdet. Dipyridamol fremkaldte også en markant stigning i plasmaets cAMP indhold.
De opnåede resultater støtter antagelsen, at dipyridamol via en forstærket effekt af endogen dannet adenosin hæmmer lipolysen og stimulerer glycolysen hos grise. Den stærkere metaboliske effekt af adenosin påvist hos Ada 0 grise er måske en del af forklaringen på, hvorfor denne type gris er særlig tilbøjelig til at udvikle dårlig kødkvalitet.
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Hyldgaard-Jensen, J.F. Adenosine Deaminase and Porcine Meat Quality. Acta Vet Scand 31, 137–143 (1990). https://doi.org/10.1186/BF03547554
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DOI: https://doi.org/10.1186/BF03547554