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Adenosine Deaminase and Porcine Meat Quality

II. Effects of Adenosine Analogues on Plasma Free Fatty Acids, Glucose and Lactate in Pigs Representing High and Low Adenosine Deaminase Red Cell Activity

Adenosin deaminase og kødkvalitet hos svin: Effekt af adenosinanaloger på plasma frie fede syrer (FFA), glukose og laktat hos grise repræsenterende høj og lav erytrocyt adenosin deaminase aktivitet

Acta Veterinaria Scandinavica volume 31, pages 145–152 (1990)Cite this article

Abstract

The adenosine analogues 5’-(N-ethyl) carboxamidoadenosine (NECA) and N6-(phenylisopropyl) adenosine (R-PIA) were shown to differ in their effect on the plasma level of free fatty acids (FFA), glucose and lactate in pigs representing low (Ada 0) and high (Ada A) red cell adenosine deaminase activity. At the same dosage range (0.001–0.005 mg/kg) R-PIA produced a much stronger suppression of the FFA level than NECA, indicating that A1 adenosine receptors predominate in porcine adipose tissue. Pretreatment with 8-phenyltheophylline completely abolished the antilipolytic effect of both adenosine analogues. NEGA in contrast to R-PIA elevated the blood glucose concentration, suggesting that A2 adenosine receptors are involved in the stimulation of glycogenosis. This effect of NECA was not altered by a β-adrenoceptor blockade providing evidence for a direct effect of adenosine on glycogenosis. Whereas the changes in plasma FFA following NECA administration were of similar magnitude in Ada A and Ada 0 pigs, the changes in the blood glucose concentration were different in these two groups of pigs.

Sammenfatning

Som led i at forklare den fundne sammenhæng mellem det polymorfe enzymsystem adenosin deaminase (Ada) og svinets kødkvalitet er en mulig betydning af adenosin for fedt- og glukosemobiliseringen søgt underbygget. Landracegrise repræsenterende høj (Ada A) og lav (Ada 0) erytrocyt Ada aktivitet tilførtes via kateter indlagt i V. jugularis to forskellige adenosin analoger (5’-N-ethylcarboxamidoadenosin, NECA og N6-phenylisopropyl-adenosin, R-PIA) for at belyse effekten af adenosin på blodets indhold af FFA, glukose og laktat. Med 30 min interval infunderedes to doser (0,001 og 0,005 mg/kg) af NECA og R-PIA på et tidspunkt, hvor de fastede grise viste en stigende og faldende hastighed af henholdsvis lipolysen og glykogenolysen. Forsøgene blev gentaget hvor der forud for NECA infusionen tilførtes dels en adenosin antagonist (8-phenyltheophyllin) og dels en β-adrenoceptor blocker (propranolol). Resultaterne viste at R-PIA ved samme dosering fremkalder et betydeligt kraftigere og længerevarende fald i plasma FFA end NECA. Det omvendte forhold sås for plasma glukose, hvor NECA fremkaldte en distinkt stigning, medens R-PIA var uden effekt. Tilførsel af adenosin receptor antagonisten 8-phenyltheophyllin ophævede fuldstændigt effekten af NECA på plasma glukoseindholdet. Tilførsel af β-blockeren propranolol forud for NECA tilførslen ændrede ikke den NECA fremkaldte stigning af plasma glukoseindholdet. Resultaterne tyder på, at adenosin in vivo kan virke som en regulator af såvel FFA- som glukoseomsætningen hos svin. Virkningen synes for lipolysens vedkommende at ske via A1 typen af adenosinreceptorer og for glykogenolysens vedkommende via A2 typen.

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Authors and Affiliations

  1. Department of Anatomy and Physiology, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1870, Copenhagen, Frederiksberg C, Denmark

    J. F. Hyldgaard-Jensen

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  1. J. F. Hyldgaard-Jensen
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Hyldgaard-Jensen, J.F. Adenosine Deaminase and Porcine Meat Quality. Acta Vet Scand 31, 145–152 (1990). https://doi.org/10.1186/BF03547555

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Acta Veterinaria Scandinavica

ISSN: 1751-0147