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Growth Characteristics of Staphylococcus aureus and Escherichia coli in Whey from Sequentially Infected Milk
Kännetecken för tillväxt av Staphylococcus aureus och Escherichia coli i vassla från infekterad mjölk
Acta Veterinaria Scandinavica volume 31, pages 169–174 (1990)
Abstract
The growth of Staphylococcus aureus and Escherichia coli was followed in bovine whey samples which had been prepared from milk previously incubated with cultures of S. aureus or E. coli Staphylococcal strains were divided into 2 groups according to their ability to form compact or diffuse colonies on serum soft agar, which is related to the absence or presence of capsule respectively. The growth of compact staphylococci was dependent on the bulk tank milk used whereas diffuse colony forming staphylococci grew equally well in all bulk milk, also in all inoculated milk. The growth of E. coli was markedly enhanced in whey samples prepared from milk preincubated with staphylococci. However, clear growth inhibition was seen with E. coli and S. aureus strains when grown in whey prepared from milk preincubated with E. coli. Results indicate that the growth promotion of pathogens due to compositional changes in milk are of importance during the course of infection because the growth pattern on staphylococci is dependent on these compositional changes. The growth-inhibitory effects caused by E. coli may explain difficulties in isolating this organism.
Sammandrag
Förökning af Staphylococcus och Escherichia coli i bovin vasslapreparat framställt av mjölk, som tidigare inkuberats med S. aureus eller E. coli, uppföljdes. Stafylokocker indelades i två grupper beroende på deras förmåga att bilda täta eller diffusa kolonier på serum soft-agar. Denna förmåga härrör sig från- resp. närvaron av kapslar. Tillväxten hos stafylokocker med täta kolonier berodde på tankmjölk medan stafylokocker med diffusa kolonier förökade sig lika bra oberoende av om man använde tankmjölk eller ympad mjölk. Förökningen av Ε. coli förbättrades märkbart i vasslepreparat framställda ur mjölk preinkuberad med stafylokocker. En klar inhibering av tillväxten kunde emellertid observeras för E. coli och S. aureus när tillväxten skedde i vasslepreparat från mjölk preinkuberad med E. coli. Resultaten visar att en gynnsam förökning av patogener är beroende av mjölkens sammansättning. Stafylo-kockerna är ytterst beroende av förändringar i sammansättningen, speciellt viktigt är detta i samband med infekteringen p. g. a. tillväxtmönstret. De tillväxtinhiberande effekterna förorsakade av E. coli kan eventuellt förklara svårigheterna i att isolera denna organism.
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Mattila-Sandholm, T., Alivehmas, T., Kaartinen, L. et al. Growth Characteristics of Staphylococcus aureus and Escherichia coli in Whey from Sequentially Infected Milk. Acta Vet Scand 31, 169–174 (1990). https://doi.org/10.1186/BF03547558
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DOI: https://doi.org/10.1186/BF03547558