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A Preliminary Study of Uterine Derived Polymorphonuclear Cell Function in Mares with Chronic Uterine Infections
En preliminär studie av cellfunktionen hos neutrofila granulocyter från livmodern hos ston med kronisk livmoderinfektion
Acta Veterinaria Scandinavica volume 31, pages 187–192 (1990)
Abstract
From 6 mares with chronic uterine infection, polymorphonuclear neutrophils (PMNs) were obtained from the uterus. In order to recover an adequate number of viable PMNs, 0.1 % oyster glycogen was infused into the uterus as a mild irritant 12 h prior to the uterine flushing.
Phagocytosis and Chemotaxis of the uterine derived PMNs were determined. The supernatant from the uterine flushing was compared with autologous serum for its capacity as an opsonin and chemoatractant. There was a significant increase of both phagocytosis and Chemotaxis when autologous serum was used compared with the supernatant from the uterine flushing.
This study indicates that autologous serum has a greater opsonizing capacity than uterine secretion from mares with chronic uterine infection. Since all mares conceived following this study, the use of oyster glycogen was not considered to have deleterious effect on the uterine mucosa.
Sammanfattning
Från 6 ston med kronisk livmoderinfektion samlades neutrofila granulocyter från livmodern. För att få ett tillråckligt antal levande neutrofila granulocyter infunderades 0.1 %-ig oyster glycogen i livmodern, som ett svagt irritationsmedel 12 timmar före livmodersköljningen. Fagocytos och kemotaxis bestämdes hos neutrofila granulocyter från livmodern. Supernatanten från livmodersköljningen jämfördes med autologt serum avseende opsoniserande och kemotaktiska fbrmåga. Det var en signifikant ökning av fagocytos och kemotaxis när autologt serum användes jämfört med supernatanten från livmodersköljningen. Denna studie indikerar att serum har en positiv effekt på fagocytos och kemotaxis hos neutrofila granulocyter från livmodern i jämförelse med livmodersekret från ston med kronisk livmoderinfektion. Då alla ston blev dräktiga efter detta experiment ansågs användningen av oyster glykogen inte ha någon skadlig effekt på livmoderns slemhinna.
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Troedsson, M., Concha, C., Einarsson, S. et al. A Preliminary Study of Uterine Derived Polymorphonuclear Cell Function in Mares with Chronic Uterine Infections. Acta Vet Scand 31, 187–192 (1990). https://doi.org/10.1186/BF03547561
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DOI: https://doi.org/10.1186/BF03547561