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A Method for Monitoring Antibodies against Staphylococcal DNases

En metode for undersøkelse av antistoffer mot stafylokokk-DNaser

Acta Veterinaria Scandinavica volume 31, pages 223–226 (1990)Cite this article

Abstract

When small amounts of DNase produced by Staphylococcus aureus, S. intermedius or S. hyicus were added to Toluidine Blue DNA Agar (TDA), a medium for demonstration of staphylococcal antiDNases was produced. By applying this medium in microtitre plates, a test system for titration of staphylococcal antibodies in serum samples was developed. A colour change from blue to pink could be observed when the DNase was allowed to act, i.e. when no staphylococcal antiDNases were present in the samples. When serum with neutralizing antibodies were applied, no colour change developed. An end-point could easily be demonstrated in dilutions of the serum. A description of the method, including certain of its limitations is given.

Sammendrag

Ved å sette små mengder DNase produsert av Staphylococcus aureus, S. intermedius eller S. hyicus til toluidinblått-DNA-agar ble det laget et medium som kunne benyttes til påvisning av stafylokokk-antiDNase. Ved å benytte dette agarmediet i mikrotiterplater ble det utviklet et testsystem for titrering av antistoffer mot stafylokokk-DNase i serumprøver. I prøver uten anti-DNaser skjedde det en fargeforandring fra blå til rosa på grunn av DNaseaktiviteten. Serumprøver med nøytraliserende antistoffer ga ingen fargeforandring. Metoden og enkelte begrensninger ved den er beskrevet.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Norwegian College of Veterinary Medicine, Oslo, Norway

    Steinar Høie & Roar Gudding

  2. National Veterinary Institute, P. O. Box 8156, N-0033, Oslo 1, Norway

    Steinar Høie & Roar Gudding

Authors
  1. Steinar Høie
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  2. Roar Gudding
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Høie, S., Gudding, R. A Method for Monitoring Antibodies against Staphylococcal DNases. Acta Vet Scand 31, 223–226 (1990). https://doi.org/10.1186/BF03547565

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  • DOI: https://doi.org/10.1186/BF03547565

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Acta Veterinaria Scandinavica

ISSN: 1751-0147