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Latex Test for Rapid Rotavirus Diagnosis in Calves

Latextest för snabb diagnos av rotavirus hos kalvar.

Acta Veterinaria Scandinavica volume 31, pages 1–4 (1990)Cite this article

Abstract

A latex agglutination test (LA) was compared with direct electron microscopy (EM) for detection of rotavirus infection in calves. A total of 375 samples from 62 calves were collected. Samples were taken when the calves were 1, 3, 5, 7, 10 and 20 days old and some scours samples were collected as well. Altogether 45/375 (12%) specimens were positive in LA and 10/375 (2.7%) were positive in EM. Samples positive in EM were also positive in LA. Out of the 62 calves studied 26/62 (42%) were positive in LA and 8/62 (13%) in EM. We found the LA very easy to perform, to be more sensitive than the EM method and probably a rather specific method for detection of rotavirus infection.

Sammendrag

En latex agglutinationstest (LA) jämfördes med direkt elektron mikroskopi (EM) för att påvisa rotavirus infektion hos kalv. Totalt 375 prov från 62 kalvar insamlades. Proven togs när kalvarna var 1, 3, 5, 7, 10 och 20 dagar gamla. Några diarreprov togs också. Allt som allt 45/375 (12%) prov var positiva med LA och 10/375 (2.7%) var positiva i EM. Alla prov som var positiva med EM var också positiva i LA. Av de 62 undersökta kalvarna var 26/62 (42%) positiva med LA och 8/62 (13%) i EM. LA-testen är enligt vår pppfattning lätt att utföra, känsligare än EM-metoden och antagligen relativt specifik för påvisning av rotavirus infektioner.

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Authors and Affiliations

  1. Department of Anatomy and Embryology, College of Veterinary Medicine, P.O. Box 6, SF-00581, Helsinki, Finland

    A. Sukura

  2. Department of Virology, National Veterinary Institute, Helsinki, Finland

    E. Neuvonen

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  1. A. Sukura
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  2. E. Neuvonen
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Sukura, A., Neuvonen, E. Latex Test for Rapid Rotavirus Diagnosis in Calves. Acta Vet Scand 31, 1–4 (1990). https://doi.org/10.1186/BF03547571

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Acta Veterinaria Scandinavica

ISSN: 1751-0147