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Phagocytosis Induced by Yeast Cells in Canine Granulocytes: A Methodological Study

Fagocytos av jästceller; en metodologisk studieavseende hundens granulocyter.

Acta Veterinaria Scandinavica volume 31, pages 87–95 (1990)Cite this article

Abstract

A phagocytic function assay of canine granulocytes was established. This method allows the proportion of active granulocytes to be estimated as well as the number of adhered and ingested yeast cells. The influence of different factors on phagocytosis was studied. Temperature variation within the interval 36–41 °C did not affect phagocytosis. The incubation time for optimal phagocytosis of yeast cells was 35 min. The opsonization procedure giving the optimal phagocytosis was purified IgG and serum together.

Sammanfattning

Avsikten med detta arbete har varit att etablera en metod för att mäta förmågan hos hundens neutrofiler att adherera och inkorporera antigen. Denna metod, där isolerade neutrofiler fagocyterar jästceller, tillåter även en bedömning av hur stor del av neutro-filpopulationen som är aktiv, dvs antingen har in-korporerat eller adhererat eller båda. Olika faktorers inverkan på de tre parametrarna undersöktes. I temperaturintervallet 36–41 °C förelåg ej någon skillnad mellan de olika temperaturerna. Opsonisering med både serum (C3b) och IgG gav en bättre aktivering och inkorporering än opsonisering med bara serum. En kinetisk studie visade att 35 min inkubering gav maximal inkkorporering. På grund av att denna metod ger en så rikhaltig information om individens granulocytfunktion är den mycket lämplig för kliniska ändamål.

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  1. Department of Medicine and Surgery, Veterinary Faculty, Swedish University of Agricultural Sciences, Uppsala, Sweden

    Gunilla Trowald-Wigh & Kerstin Thorén-Tolling

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  1. Gunilla Trowald-Wigh
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  2. Kerstin Thorén-Tolling
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Trowald-Wigh, G., Thorén-Tolling, K. Phagocytosis Induced by Yeast Cells in Canine Granulocytes: A Methodological Study. Acta Vet Scand 31, 87–95 (1990). https://doi.org/10.1186/BF03547581

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Acta Veterinaria Scandinavica

ISSN: 1751-0147