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Combined Therapeutic Effect of Dietary Selenium and Vitamin Ε on Manifested Vesd Syndrome in Weaned Pigs
Kombinerad terapeutisk effekt av dietärt selen och vitamin E på manifesterai vitamin E- och selenbrist (VESD)-syndrom hos avvanda grisar
Acta Veterinaria Scandinavica volume 19, pages 285–297 (1978)
Abstract
By using a therapeutic dietary supplementation in pigs, which had developed the vitamin Ε and selenium deficiency (VESD) syndrome, the same amounts of α-tocopheryl acetate and selenium were found to be effective as under prophylactic conditions. The experiment thus supported the conclusions that the addition of 5 mg DL-α-tocopheryl acetate/kg and 135 μg selenium/kg to a diet, which contained only traces of vitamin Ε and selenium, represents a level of minimal requirement. Glutathione peroxidase activity in blood serum was used to evaluate the selenium status in pigs. A modified method for determination of tocopherol in fat tissue was described. The addition of 15 mg α-tocopheryl acetate/kg diet was demonstrated to be sufficient to maintain the tocopherol stores in body fat at an unchanged level.
Sammanfattning
Fodret åt avvanda grisar, vilka hade insjuknat i experimentellt framkallat vitamin E- och selenbrist (VESD)-syndrom, supplemen- terades i terapeutiskt syfte med α-tokoferylacetat och selen. Samma mängder av vitamin E och selen visade sig vara verksamma vid an- vändning i terapi som i profylax. Resultaten gav således stöd åt slut- satsen att 5 mg DL-α-tokoferylacetat och 135 μg selen/kg foder, vilket ursprungligen innehåller endast spår av vitamin E och selen repre- senterar ett minimibehov av dessa ämnen för avvanda grisar. Gluta- tionperoxidas-aktiviteten i blodserum användes som ett mått på gri- sarnas selenstatus. En modifierad metod för bestämning av tokoferol i fettvävnaden beskrevs. Tillsättning av 15 mg α-tokoferylacetat/kg foder visades vara tillräckligt för att behålla kroppsfettets tokoferol- förråd på oföråndrad nivå.
References
Bengtsson, G., J. Hakkarainen, L. Jönsson, N. Lannek & P. Lindberg: A low-selenium pig diet based on casein from selenium-deficient cows. Acta vet. scand. 1974, 15, 135–137.
Bengtsson, G., J. Hakkarainen, L. Jönsson, P. Lindberg & N. Lannek: The requirement for selenium and vitamin E in weaned pigs. Proc. Int. Pig Vet. Soc. Congr. 1976, Ames, Iowa, USA p. A3.
Bengtsson, G., J. Hakkarainen, L. Jönsson, N. Lannek & P. Lindberg: Requirement for selenium (as selenite) and vitamin E (as a-to- copherol) in weaned pigs: I. The effect of varying a-tocopherol levels in a selenium deficient diet on the development of the VESD syndrome. J. Anim. Sci. 1978a, 46, 143–152.Bengtsson, G., J. Hakkarainen, L. Jönsson, N. Lannek & P. Lindberg: Requirement for selenium (as selenite) and vitamin E (as a-tocopherol) in weaned pigs: II. The effect of varying selenium levels in a vitamin E deficient diet on the development of the VESD syndrome. J. Anim. Sci. 1978b}, 46}, 153–1
Committee on Enzymes of the Scandinavian Society for Clinical Chemistry and Clinical Physiology: Recommended methods for the determination of four enzymes in blood. Scand. J. clin. Lab. Invest. 1974, 33, 291–306.
Duggan, D. E.: Spectrofluorometric determination of tocopherols. Arch. Biochem. 1959, 84, 116–122.
Ekman, L. & L.-E. Edqvist: Communication from the Department of Clinical Biochemistry, Royal Veterinary College, Stockholm, Sweden. 1974.
Ewan, R. C.: Effect of vitamin E and selenium on tissue composition of young pigs. J. Anim. Sci. 1971, 32, 883–887.
Groce, A. W., E. R. Miller, D. E. Ullrey, P. K. Ku, K. K. Keahey & D. J. Ellis: Selenium requirements in corn-soy diets for growingfinishing swine. J. Anim. Sci. 1973, 37, 948–956.
Hakkarainen, J., P. Lindberg, G. Bengtsson, L. Jönsson & N. Lannek: Requirement for selenium (as selenite) and vitamin E (as a-tocopherol) in weaned pigs: III. The effect on the development of the VESD syndrome of varying selenium levels in a low- tocopherol diet. J. Anim. Sci. 1978a, 46, No. 4. In press.
Hakkarainen, J., P. Lindberg, G. Bengtsson & L. Jönsson: Serum glutathione peroxidase activity and blood selenium in pigs. Acta vet. scand. 1978b, 19, 269–284.
Horwitt, M. K., C. C. Harvey, C. H. Dahm Jr. & M. T. Searcy: Relationship between tocopherol and serum lipid levels for determination of nutritional adequacy. Ann. N. Y. Acad. Sci. 1972, 203, 223–236.
Lindberg, P.: Determination of tocopherols in cereals by thin-layer chromatography. Acta agric. scand. 1966, 16, 217–220.
Lindberg, P.: Selenium determination in plant and animal material, and in water. Acta vet. scand. 1968, 9, Suppl. 23, 48 pp.
Little, C., R. Olinescu, K. G. Reid & P. J. O'Brien: Properties and regulation of glutathione peroxidase. J. biol. Chem. 1970, 245, 3632–3636.
Paglia, D. E. & W.N. Valentine: Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. clin. Med. 1967, 70, 158–169.
Piper, R. C., J.A. Froseth, L. R. McDowell, G. H. Kroening & I.A. Dyer: Selenium-vitamin E deficiency in swine fed peas (Pisum sativum). Amer. J. vet. Res. 1975, 36, 273–281.
Sharp, B. A., L. G. Young & A. A. van Dreumel: Dietary induction of mulberry heart disease and hepatosis dietetica in pigs. I. Nutritional aspects. Ganad. J. comp. Med. 1972a, 36, 371–376.
Sharp, B.A., L.G. Young & A. A. van Dreumel: Effect of supplemental vitamin E and selenium in high moisture corn diets on the incidence of mulberry heart disease and hepatosis dietetica in pigs. Ganad. J. comp. Med. 1972b, 36, 393–397.
Sharp, B.A., A. A. van Dreumel & L. G. Young: Vitamin E, selenium and methionine supplementation of dystrophogenic diets for pigs. Ganad. J. comp. Med. 1972c, 36, 398–402.
Trapp, A.L., K. K. Keaheg, D. L. Whitenack & C. K. Whitehair: Vitamin E —selenium deficiency in swine: Differential diagnosis and nature of field problem. J. Amer. vet. med. Ass. 1970, 157, 289–300.
Van Vleet, J. F., K. B. Meger, H. J. Olander & G. R. Ruth: Efficacy and safety of selenium vitamin E injections in newborn pigs to prevent subclinical deficiency in growing swine. Amer. J. vet. Res. 1975, 36, 387–393.
Wastell, M. E.: The role of vitamin E and selenium in the nutrition of the pig. Diss. Abstr. Int. B. 1970, 31, 1633.
Wretlind, B., K. Orstadius & P. Lindberg: Transaminase and transferase activities in blood plasma and in tissues of normal pigs. Zbl. Vet.-Med. 1959, 6, 963–970.
Acknowledgment
We are indebted to Mrs. Ann-Margret Beskow, to Mrs. Eva Kikas and to Mr. Filip Carlson for their excellent technical assistance.
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Hakkarainen, J., Lindberg, P., Bengtsson, G. et al. Combined Therapeutic Effect of Dietary Selenium and Vitamin Ε on Manifested Vesd Syndrome in Weaned Pigs. Acta Vet Scand 19, 285–297 (1978). https://doi.org/10.1186/BF03547633
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DOI: https://doi.org/10.1186/BF03547633