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Enzyme-Linked Immunosorbent Assay (Elisa) for Detection of Antibodies to Mycobacterium Para-Tuberculosis in Cattle

Abstract

Paratuberculosis may be diagnosed by clinical, bacteriological and immunological methods, but so far only the demonstration of M. paratuberculosis is considered a definite proof of the infection. World-wide use is being made of the complement fixation (CF) test as a valuable immunological test for diagnosis of clinical cases, but its low specificity and sensitivity makes its value problematic in non-clinical cases.

References

  • Engivall, E. & P. Perlmann: Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 1971, 8, 871–874.

  • Jensen, M. H.: A complement-fixation test for Johne’s disease in cattle. Nord. Vet.-Med. 1956, 8, 357–367.

  • Nassau, E., E. R. Parsons & G. D. Johnson: The detection of antibodies to Mycobacterium tuberculosis by microplate enzyme-linked immunosorbent assay (ELISA). Tubercle (Edinb.). 1976, 57, 67–70.

  • Ruitenberg, E. J., P. A. Steerenberg, B. J. M. Brosi & J. Buys: Reliability of the enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of Trichinella spiralis infections in conventionally raised pigs. T. Diergeneesk. 1976, 101, 57–70.

  • Schuurs, A.H.W.M. & B. K. Van Weemen: Enzyme-immunoassay (Review). Clin. chim. Acta. 1977, 81, 1–40.

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Jørgensen, J.B., Jensen, P.T. Enzyme-Linked Immunosorbent Assay (Elisa) for Detection of Antibodies to Mycobacterium Para-Tuberculosis in Cattle. Acta Vet Scand 19, 310–312 (1978). https://doi.org/10.1186/BF03547635

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  • DOI: https://doi.org/10.1186/BF03547635