- Published:
The Effect of pH and Potassium Phosphate Buffer on the Toxicity of Cadmium for Bacteria
Inverkan av pH och kaliumfosfatbuffer på toxisiteten av kadmium för bakterier
Acta Veterinaria Scandinavica volume 19, pages 93–101 (1978)
Abstract
An increase in the pH of Plate Count Agar led to increased toxicity of cadmium (Cd) for Micrococcus luteus ATCC 9341, Staphylococcus aureus ATCC 12600, Clostridium perfringens ATCC 8798, Escherichia coli ATCC 11775 and Pseudomonas aeruginosa ATCC 10145. The effect of the pH on toxicity was not clearly observed with Streptococcus bovis ATCC 9809 and was absent with Bacillus subtilis ATCC 8473.
When potassium phosphate buffer was added to the growth medium, the Cd toxicity for M. luteus and B. subtilis was enhanced. The toxicity of Cd for S. bovis decreased when potassium phosphate buffer was added to the medium. When the pH increased at differing phosphate concentrations, the sensitivity of M. luteus to Cd decreased.
In the authors’ opinion, the effect of pH on Cd toxicity for bacteria is due to a continuously increasing negative charge towards an alkaline value by most bacteria which increases the affinity of cations towards the cell wall.
Sammanfattning
Toxisiteten av kadmium (Gd) för Micrococcus luteus ATGG 9341, Staphylococcus aureus ATCG 12600, Clostridium perfringens ATCG 8798, Escherichia coli ATGG 11775 och Pseudomonas aeruginosa ATCG 10145 ökade vid stigande pH i bakterieräkningsmediet. Inverkan av pH på toxisiteten var mindre utpräglad för Streptococcus bovis ATCC 9809 och utblev för Bacillus subtilis ATCG 8473. När kaliumfosfatbuffer tillsattes i odlingsmediet tilltog toxisiteten av Gd för M. luteus och B. subtilis men minskade för S. bovis. När pH-värdet i mediet steg vid olika fosfatkonsentrationer minskade känsligheten av M. luteus för Gd. Författarna är av den åsikten att effekten av pH på toxisiteten av Gd är för de flesta mikrober förorsakad av den ökade negativa laddningen vid tilltagande alkalisitet, vilket ökar cellväggens affinitet för kationer.
References
Abelson, P. H. & E. Aldous: Ion antagonisms in microorganisms. Interference of normal magnesium metabolism by nickel, cobalt, cadmium, zinc, and manganese. J. Bact. 1950, 60, 401–413.
Babich, H. & G. Stotzky: Sensitivity of various bacteria, including actinomycetes, and fungi to cadmium and the influence of pH on sensitivity. Appl. Environ. Microbiol. 1977, 33, 681–695.
Baird-Parker, A. C.: Genus I. Micrococcus. In Buchanan, R. E. & N. E. Gibbons, eds.: Bergey's Manual of Determinative Bacteriology, 8th Ed. The Williams & Wilkins Company, Baltimore, USA 1974, 478–483.
Barber, M. & P. M. Waterworth: Penicillinase-resistant penicillins and cephalosporins. Brit. med. J. 1964, 2, 344–349.
Bernheim, F.: The effect of sulfhydryl compounds and mercurials on the swelling rate of cells of Pseudomonas aeruginosa in sodium and potassium buffers. Proc. Soc. exp. Biol. (N.Y.) 1970, 133, 841–844.
Bernheim, F.: Effect of Na salts of organic acids on acid induced swelling and K loss in Pseudomonas aeruginosa and effect of pH on actions of mercuric, cadmium and zinc salts. Cytobios 1973, 8, 7–13.
Donald, C., B. I. Passey & R. I. Swaby: A comparison of methods for removing trace metals from microbiological media. J. gen. Microbiol. 1952, 7, 211–220.
Friberg, L., M. Piscator, G. F. Nordberg & T. Kjellström: Cadmium in the Environment, CRC Press, Cleveland, USA 1974.
Gibson, T. & R. E. Gordon: Genus I. Bacillus. In Buchanan, R. E. & N. E. Gibbons, eds.: Bergey's Manual of Determinative Bacteriology. 8th Ed. The Williams & Wilkins Company, Baltimore, USA 1974, 529–550.
Hahne, H. C. H. & W. Kroontje: Significance of pH and chloride concentration on behavior of heavy metal pollutants: mercury(II), cadmium(II), and lead(II). J. Environ. Qual. 1973, 2, 444–450.
Heptinstall, S., A. R. Archibald & J. Baddiley: Teichoic acids and membrane function in bacteria. Nature (Lond.) 1970, 225, 519–520.
Kondo, I., T. Ishikawa & H. Nakahara: Mercury and cadmium resistances mediated by the penicillinase plasmid in Staphylococcus aureus. J. Bact. 1974, 117, 1–7.
Lamanna, C., M. F. Mallette & L. N. Zimmerman: Basic Bacteriology — its Biological and Chemical Background. 4th Ed. The Williams & Wilkins Company, Baltimore, USA 1973.
Miller, J. E., J. J. Hassett & D. E. Koeppe: Uptake of cadmium by soybeans as influenced by soil cation exchange capacity, pH, and available phosphorus. J. Environ. Qual. 1976, 5, 157–160.
Mitchell, P.: Transport of phosphate across the surface of Micrococcus pyogenes: Nature of the cell 'inorganic phosphate'. J. gen. Microbiol. 1953, 9, 273–287.
Nakahara, H., T. Ishikawa, Y. Sarai, I. Kondo, H. Kozukue & S. Silver: Linkage of mercury, cadmium and arsenate and drug resistance in clinical isolates of Pseudomonas aeruginosa. Appi. Environ. Microbiol. 1977, 33, 975–976.
Neraal, R. & R. Hamm: Methode zur quantitativen Bestimmung von zugesetztem Diphosphat und Tripolyphosphat in zerkleinertem Fleisch. (Method for quantitative determination of added diphosphate and tripolyphosphate in comminuted meat). Die Fleischwirtschaft 1972, 52, 1171–1174.
Novick, R. P. & C. Roth: Plasmid-linked resistance to inorganic salts in Staphylococcus aureus. J. Bact. 1968, 95, 1335–1342.
Ramamoorthy, S. & D. J. Kushner: Binding of mercuric and other heavy metal ions by microbial growth media. Microb. Ecol. 1975, 2, 162–176.
Tokuyama, T. & K. Asano: Studies on cadmium-ion-resistant bacteria. I. Pure isolation and bacteriological characteristics. Bull. Coll. agrie, vet. Med. Nihon Univ. 1974, 31, 182–190.
Tynecka, Z., J. Zajac & Z. Gos: Plasmid dependent impermeability barrier to cadmium ions in Staphylococcus aureus. Acta Microbiol, polon. Ser. A 1975, 7, 11–20.
Williams, C. H. & D. J. David: The accumulation in soil of cadmium residues from phosphate fertilizers and their effect on the cadmium content of plants. Soil Sci. 1976, 121, 86–93.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Korkeala, H., Pekkanen, T.J. The Effect of pH and Potassium Phosphate Buffer on the Toxicity of Cadmium for Bacteria. Acta Vet Scand 19, 93–101 (1978). https://doi.org/10.1186/BF03547645
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1186/BF03547645