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Pubertal Development of Intersertoli Cell Junctions in the Testis of Corriedale Ram Lambs

Utvecklingen under puberteten av junctions mellan sertoliceller i testiklarna hos corridale bagglamm

Acta Veterinaria Scandinavica volume 36, pages 543–551 (1995)Cite this article

Abstract

The ultrastructure of the tight junctional complex of pubertal ram lamb Sertoli cells was studied in immersion-fixed samples and related to clinical data and a light microscopical classification of the degree of spermatogenesis attained in the corresponding seminiferous tubule. Although the process followed the general mammalian developmental trend for tight junction complex formation, 2 unusual ultrastructural features were detected: the presence of an active Golgi complex during the early stage of tight junction formation and the transitory presence of ribosomes on both faces of the ectoplasmic cisternae bordering the developing junctions. The significance of these findings is discussed.

Sammanfattning

Ultrastrukturen av »tight junctional complex« i Sertoli celler hos icke könsmogna bagglamn studerades i immersions fixerade prov. De ultrastrukturella fynden relaterades till kliniska data och ljus mikroskopisk klassifikation av graden av spermatogenes i intilliggande tubuli seminiferi. Fastän processen följde den generella utvecklingstrenden hos däggdjur för bildandet av »tight junctional complex«, upptäcktes två ovanliga ultrastrukturella egenheter; närvaron av ett aktivt Golgi komplex under det tidiga stadiet av uppbyggnaden av »tight junctions och den övergående närvaron av ribosomer på båda sidor av de ektoplasmatiska cisternerna som gränsar till de »junetions« som bildas. Betydelse av dessa fynd diskuteras.

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Acknowledgments

The authors thank Mrs M. Ekwall for excellent technical assistance in preparing material for electron microscopy. Partial financial support was received by the Swedish University of Agricultural Sciences, the Swedish Council for Forestry and Agricultural Research and the Swedish Agency for Research Cooperation with Developing Countries (SAREC). Dr. Bi-elli is holder of a guest scholarship from the Swedish Institute, Stockholm and financial support from the Universidad Mayor de la República, Uruguay.

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Authors and Affiliations

  1. Departments of Anatomy and Histology, Obstetrics and Gynaecology, Uppsala, Sweden

    A. Bielli & H. Rodriguez-Martinez

  2. Faculty of Veterinary Medicine, Obstetrics and Gynaecology, Uppsala, Sweden

    A. Castrillejo

  3. Depts. of Histology & Embriology, Pathology, Uruguay

    A. Bielli & T. Gastel

  4. Faculty of Veterinary Medicine, Pathology, Uruguay

    A. Moraña

  5. Dept. of Obstetrics & Gynaecology, Swedish University of Agricultural Sciences, P.O. Box 7039, S-750 07, Uppsala, Sweden

    A. Moraña

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  1. A. Bielli
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  2. T. Gastel
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  4. A. Moraña
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  5. H. Rodriguez-Martinez
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Bielli, A., Gastel, T., Castrillejo, A. et al. Pubertal Development of Intersertoli Cell Junctions in the Testis of Corriedale Ram Lambs. Acta Vet Scand 36, 543–551 (1995). https://doi.org/10.1186/BF03547668

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Acta Veterinaria Scandinavica

ISSN: 1751-0147