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A Comparative Study of Staphylococcus aureus Strains Isolated from Bovine Subclinical Mastitis During 1952–1956 and 1992

Abstract

Fiftytwo strains of S. aureus isolated from cases of bovine subclinical mastitis in 52 different dairy herds in Denmark, in the periods 1952 to 1956 and 1992, were compared with regard to their phage- and EcoRI ribo-types. Furthermore, susceptibility to penicillin and production of fibrinolysin were used as additional phenotypic markers. Fortynine strains (94%) could be separated into 12 phage types. Ribotyping assigned the 52 strains to 21 different types. Both methods showed that 57% of the 1950’s strains and between 38–45% of the 1992 strains belonged to 3 dominating types. The remaining strains were placed by ribotyping in 8 types occurring among the 1952–1956 strains and 10 types occurring among the 1992 strains. In 87% of the strains the results of the 2 typing methods were in accordance. However, 7 strains gave different results by the 2 methods including 2 strains with major differences. Penicillin resistance only occurred in a single genotype from the 1950’s compared to 6 different genotypes among the 1992 strains.

References

  • Anonymous: Progress in mastitis control. Bull. no. 187. International Dairy Federation 1985.

    Google Scholar 

  • Anonymous: Bovine mastitis. Definition and guidelines for diagnosis. Bull. no. 211. International Dairy Federation 1987.

    Google Scholar 

  • Ausubel FM, Brent R, Kingston RE {etet al.}: Short protocols in molecular biology, (2nd ed) Greene Publishing Associates and John Wiley and Sons, New York. 1989, pp 2–11.

    Google Scholar 

  • Blair JW, Williams REO: Phagetyping of Staphylococci. Bull. Wld. Hlth. Org., 1961, 24, TH–78A.

    Google Scholar 

  • Blumberg HM, Rimland D, Kiehlbauch JA, Terry PM, Wachsmuth IK: Epidemiologic typing of Staphylococcus aureus by DNA restriction fragment length polymorphisms of rRNA genes: Elucidation of the clonal nature of a group of bacteriophage-non typeable, ciprofloxacin-resistant, methicillin-susceptible S. aureus isolates. J. Clin. Microbiol., 1992, 30, (362–369).

    Article  CAS  Google Scholar 

  • Booth JM: Update on mastitis: I. Control measures in England and Wales. How have they influenced incidence and aetiology? Br. Vet. J., 1988, 144, (316–322).

    CAS  PubMed  Google Scholar 

  • Bramley AJ, Dodd FH: Reviews of the progress of dairy science: Mastitis control - progress and prospects. J. Dairy Res., 1984, 57, (481–512).

    Article  Google Scholar 

  • Casals JB, Pringler N: Antibacterial sensitivity testing using Neo-Sensitabs. Rosco Diagnostica: Taastrup, Denmark, 1991.

    Google Scholar 

  • Davidson I: Observations on the pathogenic Staphylococci in a dairy herd during a period of six years. Res. vet. Sei., 1961, 2, (22–40).

    Article  Google Scholar 

  • Davidson I: A collaborative investigation of phages for typing bovine Staphylococci. Bull. Wld. Hlth. Org., 1972, 46, (81–98).

    Google Scholar 

  • De Buyser ML, Morvan A, Aubert S, Dilasser F, El Solh N: Evaluation of a ribosomal RNA gene probe for the identification of species and subspecies within the genus Staphylococcus. J. Gen. Microbiol., 1992, 138, (889–899).

    Article  Google Scholar 

  • Devriese LA, Van De Kerckhove A: A comparison of methods used for testing staphylokinase (fibrinolysin) Produktion in Staphylococcus strains. Antonie van Leeuwenhoek 1980, 46, (457–465).

    Article  CAS  Google Scholar 

  • Dodd FH: Progress in mastitis control. Kieler Milch-wirtschaftliche Forschungsberichte 1985, 37, (216–223).

    Google Scholar 

  • Fox LK, Gershman M, Hanock DD, Hutton CT: Fomites and reservoirs of Staphylococcus aureus causing intramammary infections as determined by phage typing: the effect of milking time hygiene practices. Cornell Vet., 1991, 81, (183–193).

    CAS  PubMed  Google Scholar 

  • Holmberg O: Phagetyping of Staphylococcus aureus strains isolated in Sweden from bovine milk. Acta vet. scand., 1975, 16, (411–419).

    Article  CAS  Google Scholar 

  • Mackie DP, Pollock DA, Rodgers SP, Logan EF: Phage typing of Staphylococcus aureus associated with subclinical bovine mastitis. J. Dairy Res., 1987, 54, (1–5).

    Article  CAS  Google Scholar 

  • Parker MT: The significance of phage-typing patterns in Staphylococcus aureus. In: Easmon CSF, Adlam C (eds): Staphylococci and Staphylococcal infections. Academic Press, London, New York, Paris, San Diego, San Francisco, Sao Paulo, Sydney, Tokyo, Toronto. 1983; 33–62.

    Google Scholar 

  • Price P, Neave FK, Rippon JE, Williams REO: The use of phage typing and penicillin sensitivity tests in studies of Staphylococci from bovine mastitis. J. Dairy Res., 1954, 27, (342–353).

    Article  Google Scholar 

  • Renneberg J, Rosdahl VT: Epidemiological studies of penicillin resistance in Danish Staphylococcus aureus strains in the period 1977-1990. Scand. J. Infect. Dis., 1992, 24, (401–409).

    Article  CAS  Google Scholar 

  • Romer O: Problems of mastitis. Nord. Vet.Med., 1949, 7, (509–527).

    Google Scholar 

  • Schalm OW, Carroll EJ, Jain NC: Bovine mastitis. Lea and Febiger, Philadelphia. 1971.

    Google Scholar 

  • Smith HW: The typing of Staphylococci of animal origin by the bacteriophage method. J. Comp. Pathol., 1948, 58, (179–188).

    Article  CAS  Google Scholar 

  • Southern EM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol., 1975, 98, (503–517).

    Article  CAS  Google Scholar 

  • Zierdt CH, Robertson EA, Williams RL, MacLowry JD: Computer analysis of Staphylococcus aureus phage typing data from 1957 to 1975, citing epidemiological trends and natural evolution within the phage typing system. Appl. Environ. Microbiol., 1980, 623–629.

    Google Scholar 

Download references

Acknowledgments

We gratefully acknowledge the assistance of René Hendriksen and the staff of the Department of Microbiology. We are grateful to N.O. Klastrupt, DVM, for making the collection of the 1952–1956 strains available, to Johannes Jensen, DVM, for help in collecting the 1992 strains and to Niels Einar Jensen, DVM, for assistance in preparing the research and the manuscript.

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Aarestrup, E.M., Wegener, H.C. & Rosdahl, V.T. A Comparative Study of Staphylococcus aureus Strains Isolated from Bovine Subclinical Mastitis During 1952–1956 and 1992. Acta Vet Scand 36, 237–243 (1995). https://doi.org/10.1186/BF03547692

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  • DOI: https://doi.org/10.1186/BF03547692

Keywords

  • phage typing
  • ribotyping