Skip to main content
Download PDF
  • Published:

Antibiotic Resistance of Vibrio anguillarum, in Relation to Serovar and Plasmid Contents

Acta Veterinaria Scandinavica volume 36, pages 55–64 (1995)Cite this article

Abstract

A total of 520 Vibrio anguillarum strains, isolated from fish and the environment, were tested for their sensitivity to 20 different antibiotics. Most isolates were of European origin. The results were compared with data on the O-serogroup and plasmid contents. All strains were sensitive to neomycin, spectinomycin, nitrofurantoin, flumequine and oxolinic acid, while most strains were sensitive to streptomycin, Oxytetracycline, chloramphenicol, sulphonamides, trimethoprim, sulphonamides with trimethoprim, nalidixan, rifampicin, novobiocin and O/129. A major part of the strains were resistant to the macrolides, spiramycin and lincomycin. For ampicillin, cephalothin, and Colistin marked differences were recorded with respect to O-serogroup. Most O1 strains were resistant to Colistin and sensitive to ampicillin and cephalothin, while most O2 strains were sensitive to Colistin but resistant to ampicillin and cephalothin. Some antibiotic resistant strains carried plasmids but no conjugation experiments were carried out to detect possible R factors.

References

  • Aiderman DJ, Michel C: Chemotherapy in aquaculture today. In: Michel C & Aiderman DJ (eds.): Chemotherapy in aquaculture: from theory to reality. Off. Int. Epiz., Paris, France, 1992, pp. 3–24.

    Google Scholar 

  • Alsina M, Martmez-Picado J, Jofre J, Blanch AR: A medium for presumptive identification of Vibrio anguillarum. Appl. environ. Microbiol. 1994, 60, 1681–1683.

    Article  CAS  Google Scholar 

  • Aoki T: Present and future problems concerning the development of resistance in aquaculture. In: Michel C & Aiderman DJ (eds.): Chemotherapy in aquaculture: from theory to reality. Off. Int. Epiz., Paris, France, 1992, pp. 254–262.

    Google Scholar 

  • Aoki T, Egusa S, Arai T: Detection of R factors in naturally occurring Vibrio anguillarum strains. Antimicrob. Agents Chemother. 1974, 6, 534–538.

    Article  CAS  Google Scholar 

  • Aoki T, Jo Y, Egusa S: Frequent occurrence of drug resistant bacteria in ayu (Plecoglossus altivelis) culture. Fish Pathol. 1980, 75, 1–6.

    Article  Google Scholar 

  • Aoki T, Kanazawa T, Kitao T: Epidemiological surveillance of drug resistant Vibrio anguillarum strains. Fish Pathol. 1985, 20, 199–208.

    Article  CAS  Google Scholar 

  • Aoki T, Kitao T, Kawano K: Changes in drug resistance of Vibrio anguillarum in cultured ayu, Plecoglossus altivelis Temminck and Schlegel, in Japan. J. Fish Dis. 1981, 4, 223–230.

    Article  CAS  Google Scholar 

  • Aoki T, Kitao T, Watanabe S, Takeshita S: Drug resistance and R plasmids in Vibrio anguillarum isolated in cultured ayu (Plecoglossus altivelis). Microbiol. Immunol. 1984, 28, 1–9.

    Article  CAS  Google Scholar 

  • Casals JB, Pringler N: Antib acterial/antifungal sensitivity testing using Neo-sensitabs. Rosco Diag- nostica, Taastrup, Denmark, 1991.

    Google Scholar 

  • Crosa JH, Schiewe MH, Falkow S: Evidence for plasmid contribution to virulence of the fish pathogen Vibrio anguillarum. Infect. Immun. 1977, 18, 509–513.

    Article  CAS  Google Scholar 

  • Davidson LS, Oliver JD: Plasmid carriage in Vibrio vulnificus and other lactose-fermenting marine vibrios. Appl. environ. Microbiol. 1986, 57, 211–213.

    Article  Google Scholar 

  • Hansen PK, Lunestad BT, Samuelsen OB: Ecological effects of antibiotics and chemotherapeutics from fish farming. In: Michel C & Aiderman DJ (eds.): Chemotherapy in aquaculture: from the ory to reality. Off. Int. Epiz., Paris, France, 1992, pp. 174–178

    Google Scholar 

  • Hedges RW, Smith P, Brazil G: Resistance plasmids of aeromonads. J. gen. Microbiol. 1985, 131, 2091–2095.

    Google Scholar 

  • Hedges RW, Vialard JL, Pearson NJ, O’Grady F: R plasmids from Asian strains of Vibrio cholerae. Antimicrob. Agents Chemother. 1977, 77, 585–588.

    Article  Google Scholar 

  • van der Heijden MHT, van Muiswinkel WB, Grondel JL, Boon JH: Immunomodulating effects of antibiotics. In: Michel C & Aiderman DJ (eds.): Chemotherapy in aquaculture: from theory to reality. Off. Int. Epiz., Paris, France, 1992, pp. 219–230

    Google Scholar 

  • Inglis V, Yimer E, Bacon EJ, Ferguson S: Plasmid- mediated antibiotic resistance in Aeromonas sal- monicida isolated from Atlantic salmon, Salmo salar L., in Scotland. J. Fish Dis. 1993, 76, 593–599.

    Article  Google Scholar 

  • Kado CI, Liu ST: Rapid procedure for detection and isolation of large and small plasmids. J. Bacte- riol. 1981, 145, 1365–1373.

    Article  CAS  Google Scholar 

  • Larsen JL: Vibrio anguillarum’. characterization, ecology, and patogenicity. Dissertation. DSR Press, Copenhagen, Denmark, 1990.

    Google Scholar 

  • Larsen JL, Olsen JE: Occurrence of plasmids in Danish isolates of Vibrio anguillarum serovars O1 and 02 and association with phenotypic characteristics. Appl. environ. Microbiol. 1991, 57, 2158–2163.

    Article  CAS  Google Scholar 

  • Larsen JL, Pedersen K, Dalsgaard I: Vibrio anguillarum serovars associated with vibriosis in fish. J. Fish Dis. 1994, 77, 259–267.

    Article  Google Scholar 

  • Lunestad BT: Fate and effects of antibacterial agents in aquatic environments. In: Michel C & Aiderman DJ (eds.): Chemotherapy in aquaculture: from theory to reality. Off. Int. Epiz., Paris, France, 1992, pp. 152–161.

    Google Scholar 

  • Macrina FL, Kopecko DJ, Jones KR, Ayers DJ, McCowen KR: A multiple plasmid-containing Escherichia coli strain: Convenient source of size reference plasmid molecules. Plasmid 1978, 7, 417–420.

    Article  Google Scholar 

  • Mitoma Y, Aoki T, Crosa JH: Phylogenetic relationships among Vibrio anguillarum plasmids. Plasmid 1984, 72, 143–148.

    Article  Google Scholar 

  • Muroga K, Yoneyama N, Jo Y: Vibriostatic agent- nonsensitive Vibrio anguillarum isolated from ayu. Fish Pathol. 1979, 13, 159–162.

    Article  Google Scholar 

  • Murray PR, Zeitinger JR, Krogstad DJ: Reliability of disc diffusion susceptibility testing. Infection Control and Hospital Epidemiology 1992, 3, 230–237.

    Google Scholar 

  • Myhr E, Larsen JL, Lillehaug A, Gudding R, Heum M, Hastein T: Characterization of Vibrio anguil- larum and closely related species isolated from farmed fish in Norway. Appl. environ. Microbiol. 1991, 57, 2750–2757.

    Article  CAS  Google Scholar 

  • Samuelsen OB: The fate of antibiotics/chemothera- peutics in marine aquaculture sediments. In: Michel C & Aiderman DJ (eds.): Chemotherapy in aquaculture: from theory to reality. Off. Int. Epiz., Paris, France, 1992, pp. 162–173.

    Google Scholar 

  • Sandaa R-A, Enger O: Transfer of a broad range plasmid from Aeromonas salmonicida to bacteria indigenous to the marine environment, p. 31, Abstr. 6th Int. Conf. “Diseases of Fish and Shellfish”, Brest, France, September 5-10,1993.

  • Schlotfeldt HJ: Current practics of chemotherapy in fish culture. In: Michel C & Aiderman DJ (eds.): Chemotherapy in aquaculture: from theory to reality. Off. Int. Epiz., Paris, France, 1992, pp. 25–38.

    Google Scholar 

  • Sizemore RK, Colwell RR: Plasmids carried by antibiotic-resistant marine bacteria. Microb. Agents Chemother. 1977, 12, 373–382.

    Article  CAS  Google Scholar 

  • Sfirensen UBS, Larsen JL: Serotyping of Vibrio an- guillarum. Appl environ Microbiol 1986,57,593–597.

    Article  Google Scholar 

  • Sorum H, Kvello JH, Hastein H: Occurrence and stability of plasmids in Aeromonas salmonicida ss salmonicida isolated from salmonids with furunculosis. Dis. aquat. Org. 1993, 76, 199–206.

    Article  Google Scholar 

  • Sorum H, Poppe T, Olsvik O: Plasmids in Vibrio salmonicida isolated from salmonids with hemorrhagic syndrome (Hitra disease). J. clin. Microbiol. 1988, 26, 1679–1683.

    Article  Google Scholar 

  • Tanaka M, Hanada H, Yoshikawa M: Vibriosis of ayu in Shizuoka Prefecture, from 1984 to 1990 - serotype and drug sensitivity. Gyobyo Kenkyu 1993, 23, 77–82.

    Article  Google Scholar 

  • Threlfall EJ, Rowe B, Ferguson JL, Ward LR: Characterization of plasmids conferring resistance to gentamicin and apramycin in strains of Salmonella typhimurium phage type 204C isolated in Britain. J. Hyg. Camb. 1986, 97, 419–426.

    Article  CAS  Google Scholar 

  • Toranzo AE, Barja JL, Colwell RR, Hetrick FM: Characterization of plasmids in bacterial fish pathogens. Infect. Immun 1983a, 39, 184–192.

    Article  CAS  Google Scholar 

  • Toranzo AE, Barja JL, Potter SA, Colwell RR, Hetrick FM, Crosa JH: Molecular factors associated with virulence of marine vibrios isolated from striped bass in Chesapeake Bay. Infect. Immun 1983b, 39, 1220–1227.

    Article  CAS  Google Scholar 

  • Toranzo AE, Santos Y, Lemos ML, Ledo A, Bolinches J: Homology of Vibrio anguillarum strains causing epizootics in turbot, salmon and trout reared on the Atlantic coast of Spain. Aquaculture 1987, 67, 41–52.

    Article  Google Scholar 

  • Toranzo AE, Santos Y, Nunes S, Barja JL: Biochemical and serological characteristics, drug resistance and plasmid profiles of Spanish isolates of Aeromonas salmonicida. Gyobyo Kenkyu 1991, 26, 55–60.

    Article  CAS  Google Scholar 

  • Zhao J, Kim E, Kobayashi T, Aoki T: Drug resistance of Vibrio anguillarum isolated from ayu between 1989 and 1991. Nippon Suisan Gakkai- shi 1992, 58, 1523–1527.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The technical assistance of Miss Susanne Faaborg Nielsen and Miss Annette Rasmussen and the financial support from the Danish Agricultural and Veterinary Research Council, grant no. 13-4508-1 NMP and 13-4710-1, from the Nordic Council of Ministers, and from CEC contract number AIR-CT92-0341 is gratefully appreciated.

Author information

Authors and Affiliations

  1. Department of Veterinary Microbiology, Laboratory of Fish Diseases, The Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870, Frederiksberg C, Denmark

    K. Pedersen, T. Tiainen & J. L. Larsen

Authors
  1. K. Pedersen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Tiainen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. L. Larsen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pedersen, K., Tiainen, T. & Larsen, J.L. Antibiotic Resistance of Vibrio anguillarum, in Relation to Serovar and Plasmid Contents. Acta Vet Scand 36, 55–64 (1995). https://doi.org/10.1186/BF03547702

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03547702

Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147