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The Precipitation of Peptides and Proteins by Lignosulphonic Acids
Presipitering au peptider og proteiner med ligninsulfonsyrer
Acta Veterinaria Scandinavica volume 12, pages 572–582 (1971)
Summary
The precipitation of peptide- and protein-lignosulphonic acid complexes can be demonstrated and studied in agar plates in the form of precipitation zones. The effects on the precipitates caused by variations in incubation temperature, incubation time, concentration of the reagents and in pH in the mixture of reagents, are described, and the nature of the peptide-lignosulphonic acid bond is discussed. The central lysis zones which appeared in some precipitation zones were found to be probably caused by excess of lignosulphonic acids. The possibility of developing the agar precipitation method described as a direct micro quantitative procedure for the determination of certain lignosulphonic acids in aqueous solution is suggested.
Sammendrag
Det har vist seg mulig å demonstrere og studere presipitering av peptider og proteiner med ligninsulfonsyrer i form av presipiteringssoner i agarplater. En har beskrevet virkningen på presipitatet av variasjon i inkuberingstemperatur, inkuberingstid, konsentrasjon av reagenter og pH i blandingen av reagenter, og en har diskutert bindingen i peptid-ligninsulfonsyre-komplekset. Lysissonene som ble utviklet sentralt i noen presipiteringssoner, er trolig forårsaket av overskudd av ligninsulfonsyrer. Muligheten for å utvikle den beskrevne agar-presipiteringsmetode som en direkte mikro-kvantitativ metode for å bestemme visse ligninsulfonsyrer i vandig løsning er antydet.
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Acknowledgement
The sulphite spent liquors used in this work were kindly supplied by A/S Tofte Cellulosefabrik, Hurum, Norway.
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Nœss, B. The Precipitation of Peptides and Proteins by Lignosulphonic Acids. Acta Vet Scand 12, 572–582 (1971). https://doi.org/10.1186/BF03547725
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DOI: https://doi.org/10.1186/BF03547725