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Peptide-Lignosulphonic Acid Precipitation Zones in Agar Gel

Presipiteringssoner dannet au peptid-ligninsulfonsyre-komplekser i agar gel: En direkte mikrokvantitatiu metode for bestemmelse au peptidpresipiterende ligninsulfonsyrer i uandig løsning

A Direct Micro Quantitative Procedure for the Determination of Peptide-Precipitating Lignosulphonic Acids in Aqueous Solution

Acta Veterinaria Scandinavica volume 12, pages 583–591 (1971)Cite this article

Summary

The effects of incubation time and incubation temperature on precipitation zones of peptide-lignosulphonic acid complexes in agar plates are studied in relation to the mathematical dependence of zone diameter as a function of the concentrations of lignosulphonic acids in serial 2-fold dilutions. Optimal conditions are given for obtaining regression lines with not significant second degree coefficients under defined conditions.

A diffusion unit, proposed as a measure of peptide-precipitating lignosulphonic acids in aqueous solution, is defined. The possibility is discussed of using the described procedure as a micro quantitative method for the determination of peptide-precipitating lignosulphonic acids in aqueous solution.

Sammendrag

En har studert virkningen av inkuberingstid og inkuberingstemperatur på presipiteringssoner av peptid-ligninsulfonsyre-komplekser i agarplater i relasjon til den matematiske avhengighet av sonediameter som funksjon av konsentrasjonene av ligninsulfonsyrer i 2-fold fortynningsserie, og en har bestemt de optimale betingelser for å oppnå regresjonslinjer uten signifikante annengradsledd under definerte betingelser.

En diffusjonsenhet, foreslått som mål for peptidpresipiterende ligninsulfonsyrer i vandig løsning, er definert. Muligheten for å bruke den beskrevne framgangsmåte som mikrokvantitativ metode for bestemmelse av peptidpresipiterende ligninsulfonsyrer i vandig løsning er diskutert.

References

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Veterinary College of Norway, Oslo, Norway

    Bjørn Nœss

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  1. Bjørn Nœss
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Nœss, B. Peptide-Lignosulphonic Acid Precipitation Zones in Agar Gel. Acta Vet Scand 12, 583–591 (1971). https://doi.org/10.1186/BF03547726

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Acta Veterinaria Scandinavica

ISSN: 1751-0147