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Sphaerophorus Necrophorus a Study of 23 Strains

Sphaerophorus necrophorus. En undersøgelse af 23 stammer

Acta Veterinaria Scandinavica volume 12, pages 344–364 (1971)Cite this article

Abstract

Twenty-three strains of Sphaerophorus necrophorus, isolated from animal pathological processes, were characterized. The two biotypes of Fievez (1963) were recognized, differing in colony morphology, cell morphology, degree of hemolysis, growth modus in broth, and hemagglutination. The following characters were shared by all strains: Gram-negative, anaerobic, nonsporeforming, immotile rods, producing acid from fructose, glucose, mannose, maltose, and (variably) from galactose, but not from arabinose, xylose, lactose, saccharose, cellobiose, melezitose, adonitole, sorbitole, mannitole, and salicine. Terminal pH in glucose medium was 5.77 ± 0.17. Growth was not inhibited by 0.008 % brilliant green, but was partially inhibited by 10 % ox bile. Indole was produced. Nitrate was not reduced. A lecitinase was present. Proteolytic properties were present. Threonine was deaminated. The fatty acids: acetic, propionic, and butyric acid were produced in medium both with and without glucose.

Sammendrag

23 stammer af Sphaerophorus necrophorus isoleredes fra animalt patologisk materiale og karakteriseredes. Repræsentanter for begge biotyper (Fievez 1963) påvistes. Disse adskilte sig ved kolonimorfologi, cellemorfologi, grad af hæmolyse, væksttype i bouillon og evne til hæmagglutination. Følgende egenskaber fandtes hos alle stammer: gramnegative, anaerobe, non sporogene, ubevægelige stave, som under syredannelse metaboliserede fructose, glucose, mannose, maltose og (variabelt) galactose, men ikke arabinose, xylose, lactose, saccharose, cellobiose, melezitose, adonitol, sorbitol, mannitol og salicin. Slut pH i glucosemedium var 5,77 ± 0,17. Vaeksten hæmmedes ikke af 0,008 % brilliantgrønt, men hæmmedes partielt af 10 % oksegalde. Indol produceredes. Nitrat reduceredes ikke. En lecitinase produceredes. Proteolytiske egenskaber påvistes. Threonin deamineredes. Af lavere fede syrer produceredes såvel i glucoseholdigt som i glucosefrit medium eddikesyre, propionsyre og smørsyre.

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  1. Department of Bacteriology and Hygiene, Royal Veterinary and Agricultural University, Copenhagen, Denmark

    Bent Aalbæk

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Aalbæk, B. Sphaerophorus Necrophorus a Study of 23 Strains. Acta Vet Scand 12, 344–364 (1971). https://doi.org/10.1186/BF03547735

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Acta Veterinaria Scandinavica

ISSN: 1751-0147