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Serum Enzyme Changes in Lames with Experimentally Induced Acute Muscular Dystrophy
Forandringer i serumenzymer hos lam med eksperimentelt fremkalt muskeldystrofi
Acta Veterinaria Scandinavica volume 12, pages 365–374 (1971)
Abstract
An experiment was performed to study some serum enzyme changes taking place in artificially fed lambs made dystrophic on a skim milk ration supplemented with α-tocopherolextracted cod liver oil. In the course of two to three weeks the lambs showed highly increased serum values of aspartate- and alanine aminotransferase (AspAT = GOT and A1AT = GPT), total lactate dehydrogenase (LDH), and creatine phosphokinase (GPK). The latter enzyme proved to be the most sensitive test of myopathies, with serum values above 1000 times the normal level. A high-grade hyaline skeletal muscle degeneration was confirmed by histology. Myocardial changes were also present.
Contrary to expectations, electrophoretic separation of LDH isoenzymes in the serum of the dystrophic lambs did not reflect any increase of the prevalent muscular cathodic thermolabile fraction LDH5. The use of a so-called LDH heat stability test as an aid in clinical diagnostic work, as previously suggested, therefore does not seem to be valid in this disease. The results are discussed.
Sammendrag
Et forsøk ble utført for å undersøke noen av de enzymforandrin-ger som finner sted i serum hos lam med eksperimentelt fremkalt muskeldystrofi. Dietten besto av skummet melk og α-tokoferolekstrahert tran. I løpet av to—tre uker viste lammene en sterk stigning i serumnivået av aspartat- og alaninaminotransferase (AspAT = GOT og A1AT = GPT), total laktatdehydrogenase (LDH) og kreatinfosfokinase (CPK). Det siste enzymet viste seg å være den mest sensitive indikator på myopatiske tilstander med serumverdier over 1000 ganger det normale nivå.
En sterkt uttalt hyalin degenerasjon av skjelettmuskulaturen ble bekreftet ved histologisk undersøkelse, samtidig som forandringer i myocardiet ble påvist.
I motsetning til hva en hadde ventet, viste elektroforetisk separasjon av LDH isoenzymene i serum av dy strof iske lam ingen økning av den katodiske termolabile fraksjon LDH5, som er sterkt representert i skjelettmuskulatur. En såkalt relativ varmestabilitetstest av LDH som et diagnostisk hjelpemiddel ved muskeldystrofi hos lam, ser derfor ikke ut til å være relevant, slik som antydet i en tidligere artikkel. Mulige forklaringer er diskutert.
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Tollersrud, S. Serum Enzyme Changes in Lames with Experimentally Induced Acute Muscular Dystrophy. Acta Vet Scand 12, 365–374 (1971). https://doi.org/10.1186/BF03547736
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DOI: https://doi.org/10.1186/BF03547736