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Proteolytic Enzymes and Biological Inhibitors
Proteolytiske enzymer og biologiske inhibitorer: V. Serologisk slektskap mellom bouint trypsin og suinetrypsin og bouint α-chymotrypsin
V. Serological Relationship Between Bovine and Swine Trypsins and Bovine α-Chymotrypsin
Acta Veterinaria Scandinavica volume 12, pages 417–428 (1971)
Abstract
The serological relationship between bovine and swine trypsins, and bovine α-chymotrypsin has been studied with rabbit antisera at different stages in the immunization period. By using paper electrophoresis to distinguish between the naturally occurring inhibitors and the antienzymes in the γ-globulin fractions, combined with the casein precipitating inhibition test (electrophoretic CPI-test) it was found that at 18 days after immunization the antienzymes inhibited only the homologous enzymes. After an additional 12 and 24 days the anti- bovine trypsin also inhibited swine trypsin and α-chymotrypsin, and anti-swine trypsin inhibited bovine trypsin, while antia-chymotrypsin inhibited only the homologous enzyme. The enzyme inhibition in the heterologous systems was about 1/10 of that in the homologous systems. Similar results were obtained by applying the Kunitz test to isolated γ-globulins. The total trypsin inhibitory activity of the whole anti- bovine trypsin serum increased 50 % from the beginning to the end of the immunization period (tested on bovine trypsin).
Using the double diffusion technique, cross precipitation only occurred between anti-bovine trypsin and swine trypsin.
Acetyltrypsin (bovine) was affected by the 3 antisera in a way similar to native bovine trypsin.
The results are discussed in relation to other reports concerning the serological relationship of animal proteinases.
Sammendrag
En har undersøkt det serologiske slektskap mellom bovint trypsin og svinetrypsin og bovint a-chymotrypsin ved forskjellige trinn i immuniseringsprosessen ved å bruke antisera fremstilt på kaniner. Ved å benytte papirelektroforese for å skille mellom de naturlige forekommende inhibitorer og antienzymene, sammen med kaseinpresipitasjonhemmingsreaksjon (GPI-test) fant en at enzymene ble hemmet bare av de homologe antienzymer etter en immuniseringstid på 18 dager. Etter ytterligere 12 og 24 døgn hemmet anti-bovint trypsin også svinetrypsin og a-chymotrypsin, og anti-svinetrypsin hemmet bovint trypsin. Antia-chymotrypsin hemmet bare det homologe enzym. Hemmingen av aktiviteten i de heterologe enzymsystemer var ca. tiendeparten av hemmingen i de homologe systemer. Ved å benytte Kunitz reaksjon fikk en tilsvarende resultater ved bruk av isolerte y-globuliner. Den totale hemming av antiserum mot bovint trypsin økte med 50 % fra begynnelsen til slutten av immuniseringsprocessen. Ved agar-geldiffusjon fikk en krysspresipitasjon bare mellom anti-bovint trypsin og svinetrypsin. Acetyltrypsin (bovint) reagerte på tilsvarende måte som ordinært bovint trypsin med alle tre antisera.
En har sammenlignet resultatene med andre arbeider som angår serologisk slektskap mellom animalske proteolytiske enzymer.
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Fossum, K. Proteolytic Enzymes and Biological Inhibitors. Acta Vet Scand 12, 417–428 (1971). https://doi.org/10.1186/BF03547740
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DOI: https://doi.org/10.1186/BF03547740