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Studies of the Blood Lipid Pattern in Healthy Dairy Cows

Undersökningar av blodlipidmönstret hos friska mjölkkor

Abstract

The object was to add to the knowledge of certain blood parameters of significance for charting the lipid metabolism in dairy cows. The result may be summarized as follows:

  1. 1.

    Determination of the serum content of NEF A and free glycerol could be done on blood samples drawn in non-refrigerated tubes and centrifuged at room temperature within 45 min. Deep-freezing of serum did not affect the results of the analysis for these parameters.

  2. 2.

    A significant diurnal variation occurred for NEFA and free glycerol. The highest values were measured in the morning, when the cows had been without feed for about 12 hrs. For glycerol (but not for NEFA) the values in the afternoon, when the cows had been without feed for 3–4 hrs., were significantly higher than those taken 2 hrs. after the morning feed.

  3. 3.

    NEFA and free glycerol varied significantly with the daily yield. The NEFA content was highest at the highest level of yield, while the glycerol values were highest during the dry-period. The investigations reported under point 3, however, included no production figures during the period 21 days before to 30 days after calving.

  4. 4.

    During the first month of lactation the NEFA and glycerol values showed a great mutual parallelism. The values during the first weeks of lactation were considerably higher than during the remaining lactation periods, which in all probability is a result of inevitable underfeeding of cows with good milking predisposition.

  5. 5.

    During the first two days after calving there were very great variations in NEFA and free glycerol. If sampling had been consistently done during the first hrs. after calving, the values would probably have been higher throughout owing to the sympathicotonia which occurs at that time.

  6. 6.

    In general, blood glucose varied less than NEFA and free glycerol with the level of yield. Thus, it seems as if blood glucose is a less sensitive measure of the energy balance.

  7. 7.

    Deep-freezing of serum did not affect the values of triglycerides. No significant diurnal variation occurred for this parameter. The triglyceride values were distinctly higher during the dry-period than during lactation. No significant differences existed, however, between different production levels or during different stages of the dry-period.

Sammanfattning

Avsikten var att komplettera kännedomen om vissa blodparametrar, vilka är av betydelse för kartläggning av fettmetabolismen hos mjölkkor. Av undersökningen framgick:

  1. 1.

    Bestämningar av serunihalten icke förestrade fettsyror (NEFA) och fri glycerol kunde med tillfredsställande noggrannhet utföras på blodprov som togs i icke kylda rör och som centrifugerats vid rumstemperatur inom 45 minuter. Djupfrysning av serum påverkade inte analysresultaten för dessa parametrar.

  2. 2.

    Signifikant dygnsvariation förelåg för NEFA och fri glycerol. De högsta värdena uppmättes före morgonutfodringen, då korna varit utan foder c:a 12 timmar. För fri glycerol (men ej för NEFA) var värdena före eftermiddagsutfodringen, då korna varit utan foder 3—4 timmar, signifikant högre än 2 timmar efter morgonutfodringens början.

  3. 3.

    NEFA- och fri glycerol varierade signifikant med dygnsavkastningen. För NEFA förelåg de högsta värdena vid den högsta produktionsnivån, medan de högsta glycerolvärdena uppmättes under sintiden. Vid de undersökningar som redovisas under 3. medtogs dock inga produktionssiffror under tiden 21 dagar före till 30 dagar efter kalvningen.

  4. 4.

    Under den första månaden efter kalvningen visade NEFA- och glycerolvärdena stor inbördes parallellitet. Värdena under de första laktationsveckorna var betydligt högre än under Övriga laktationsperioder, vilket tolkas som en följd av en oundviklig underutfodring hos kor med goda mjötkanlag.

  5. 5.

    Under de båda första dagarna efter kalvningen förelåg mycket stora variationer i NEFA- och glycerolvärden. Det antages att konsekvent provtagning i mer omedelbar anslutning till partus hade givit genomgående högre värden till följd av då föreliggande sympaticotoni.

  6. 6.

    Blodgiv koshalten varierade i allmänhet mindre med avkastningsnivån än vad som gällde för NEFA och fri glycerol. Med anledning härav drages slutsatsen att blodglykos utgör ett mindre känsligt mått på energibalansen.

  7. 7.

    Djupfrysning av serum påverkade inte triglyceridhalten. Signifikant dygnsvariation förelåg inte för serumtriglycerider, Triglyceridvärdena var entydigt högre under sintiden än när korna mjölkade. Däremot förelåg inga skillnader mellan olika produktionsnivåer eller under olika stadier av sinperioden.

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Pehrson, B. Studies of the Blood Lipid Pattern in Healthy Dairy Cows. Acta Vet Scand 12, 230–242 (1971). https://doi.org/10.1186/BF03547755

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