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Phenoxy-Acid-Induced Renal Changes in the Chicken
Fenoxiättiksgreframkallade njurförändringar hos kycklingar: I. Ultrastruktur
I. Ultrastructure
Acta Veterinaria Scandinavica volume 12, pages 243–256 (1971)
Abstract
Forty-five day-old broiler chicks were equally divided into 3 groups, 2 being fed 2,4-D and 2,4,5-T, respectively, from the fifth day of life for up to 7 months, at 1,000 p.p.m. in the drinking water, and the third serving as a control. From 8 weeks of age 2 of the control animals were fed 2,4-D at the same rate for 7 months.
Seven of the treated animals died, or were killed in a moribund state, during the experiment (Table 1), the survivors showing only slight signs of poisoning such as reduced mobility and food and water intakes.
Animals were sacrificed after varying time intervals (Table 1). The tissue distribution patterns of 2,4-D and 2,4,5-T, as determined in selected tissues of a few of the animals (Table 2), were similar to that observed earlier with 2,4-D.
Both compounds produced qualitatively similar morphological effects, although the action of 2,4,5-T apparently was somewhat more intense.
The predominant necropsy finding was kidney enlargement (Table 1) due to hypertrophy of the proximal convoluted tubular epithelium (Fig. 1).
Electron-microscopically, increased numbers of mitochondria were demonstrated in the tubular cells, with variations in mitochondrial size, shape and structure (Figs. 4–7). The number of micro-bodies was also definitely increased (Figs. 2 and 4). In the nuclei nuclear bodies were observed (Figs. 2 and 3).
The significance of the findings is discussed.
Sammanfattning
Fyrtiofem daggamla broilerkycklingar fördelades på tre grupper, varav två tillfördes fenoxiättiksyrederivat (2,4-D resp. 2,4,5-T; 1.000 p.p.m. i dricksvattnet) från femte levnadsdagen och under upp till sju månader och den tredje tjänade som kontroll. Från åtta veckors ålder tillfördes även två av kontrolldjnren 2,4-D på samma sätt under sju månader.
Sju av de behandlade djuren dog, eller avlivades i döende tillstånd, under försöket (Tabell 1). De överlevande visade lindriga förgiftningssymtom, såsom nedsatt rörlighet och minskade foder- och vattenintag.
Djuren avlivades efter varierande försökstider (Tabell 1). Vävnadshalterna av 2,4-D och 2,4,5-T, bestämda vid några tillfällen (Tabell 2), antydde distributionsmönster av samma typ som tidigare påvisats för 2,4-D.
Både föreningarna framkallade morfologiska förändringar av likartad typ, men 2,4,5-T syntes vara något mera aktivt.
Obduktionsbilden dominerades av njurförstoring (Tabell 1), betingad av epitelhypertrofi i proximala tubuli contortae (Fig. 1), Elektronmikroskopiskt påvisades i tubuliepitelet förökning av mitokondrierna med storleks-, form- och strukturförändringar (Fig. 4—7). Dessutom förekom microbodies i starkt förhöjt antal (Fig. 2 och 4). T cellernas kärnor iakttogs nuklearkroppar (Fig. 2 och 3).
Resultaten diskuteras.
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Björklund, NE., Erne, K. Phenoxy-Acid-Induced Renal Changes in the Chicken. Acta Vet Scand 12, 243–256 (1971). https://doi.org/10.1186/BF03547756
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DOI: https://doi.org/10.1186/BF03547756