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Disc Electrophoretic Studies on an S-Carboxymethylkerateine Preparation from Bovine and Canine Hair
Acta Veterinaria Scandinavica volume 12, pages 307–309 (1971)
Abstract
Reduced and iodoacetate alkylated keratin, S-carboxymethyl-kerateine (SCMK), may be separated in components differing in molecular weight and sulphur content (Gillespie et al. 1962). Species differentiation could be accomplished by moving boundary electrophoresis of high-sulphur kerateines, \({\text{S}}_{20,{\text{w}}}^{\text{O}}\) 1.55-1.65 (Gillespie 1963, Gillespie & Inglis 1965). Shechter et al. (1969) observed species differences in the acrylamide electrophero-grams of low-sulphur kerateines, mol. w. 45,000-50,000.
References
Gillespie, J. M., I. J. O'Donnell & E. O. P. Thompson: The interaction between high- and low-sulphur proteins extracted from a-keratin. Aust. J. biol. Sci. 1962, 15, 409–412.
Gillespie, J. M.: The isolation and properties of some soluble proteins from wool. Aust. J. biol. Sci. 1963, 16, 241–251.
Gillespie, J. M. & A. S. Inglis: A comparative study of high-sulphur proteins from a-keratins. Comp. Biochem. Physiol. 1965, 15, 175–185.
Shechter, Y., J. W. Landau & V. D. Newcomer: Comparative disc electrophoresis of hair kerateines. J. invest. Dermatol. 1969, 52, 57–62.
Acknowledgments
The Carlsberg Breweries Research Laboratory kindly serviced the analytical ultracentrifugations. The author is grateful to Miss Bente Thorsen for skilled technical assistance.
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Simonsen, H. Disc Electrophoretic Studies on an S-Carboxymethylkerateine Preparation from Bovine and Canine Hair. Acta Vet Scand 12, 307–309 (1971). https://doi.org/10.1186/BF03547764
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DOI: https://doi.org/10.1186/BF03547764