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Experimental Infection of Lambs with an Equine Granulocytic Ehrlichia Species Resembling the Agent that Causes Human Granulocytic Ehrlichiosis (HGE)
Acta Veterinaria Scandinavica volume 39, pages 491–497 (1998)
Abstract
Five lambs were inoculated with a granulocytic Ehrlichia species originally isolated from a Swedish horse with granulocytic ehrlichiosis (EGE). The 16S rRNA gene sequence of the Swedish Ehrlichia sp. causing EGE was identical to the sequence of the agent causing human granulocytic ehrlichiosis (HGE). After the inoculation, infected neutrophils and a low serologic response were seen in all lambs, but no clinical symptoms were observed. In one lamb 17% of the neutrophils were infected without a corresponding fever. Six weeks later the lambs were inoculated with an ovine isolate of E. phagocytophila. After challenge with E. phagocytophila the lambs reacted with fever and infected granulocytes. The results presented herein show that the equine Ehrlichia isolate was infective for lambs but generated weak immune response and no distinctive protection from subsequent challenge with E. phagocytophila.
References
Artursson K, Gunnarsson A, Wikstrom U-B, Olsson Engvall E: A serological and clinical follow-up in horses with confirmed equine granulocytic ehrliciosis (submitted for publication).
Bakken JS, Krueth J, Tilden RL, Dumler JS, Kristiansen BE: Serological evidence of human granulocytic ehrlichiosis in Norway. Eur. J. clin. Microbiol. Infect. Dis. 1996, 15, 829–832.
Barlough JE, Madigan JE, De Rock E, Dumler JS, Bakken JS: Protection against Ehrlichia equi is conferred by prior infection with the human granulocytotropic ehrlichia (HGE agent). J. clin. Microbiol. 1995, 33, 3333–3334.
Bjöersdorff A, Christenson D, Johnsson A, Sjöström AC, Madigan JE: Ehrlichia equi-infektion diagnostiserat hos hast. (Granulocytic ehrlichiosis in the horse - the first verified case in Sweden). Svensk Vet.-Tidn. 1990, 42, 357–360.
Büscher G, Gandras R, Apel G, Friedhoff KT: Der erste Fall von Ehrlichiose beim Pferd in Deutschland (Kurzmitteilung). (The first case of ehrlichiosis in a horse in Germany). Dtsch. tierärztl. Wschr. 1984, 91, 408–409.
Chen S-M, Dumler JS, Bakken JS, Walker DH: Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. J. clin. Microbiol. 1994, 32, 589–595.
Dumler JS, Asanovich KM, Bakken JS, Richter P, Kimsey R, Madigan JE: Serologic cross-reactions among Ehrlichia equi, Ehrlichia phagocytophila, and human granulocytic ehrlichia. J. clin. Microbiol. 1995, 33, 1098–1103.
Dumler JS, Dotevall L, Gustafson R, Granström M: A population-based seroepidemiologic study of human granulocytic ehrlichiosis and Lyme borreliosis on the west coast of Sweden. J. infect. Dis. 1997, 775, 720–722.
Egenvall AE, Bjöersdorff AI, Lilliehöök L, Olsson Engvall E, Karlstam E, Artursson K, Hedhammar Â, Gunnarsson A: Early manifestations of granulocytic ehrlichiosis in dogs inoculated experimentally with a Swedish Ehrlichia species isolate. Vet. Rec. 1998, 143, 412–417.
Eriksen L, Hansen JF, Abildtrup E, Olsson Engvall E: Granulocytaer ehrlichiose hos hest pavist i Danmark. (Equine granulocytic ehrlichiosis diagnosed in Denmark). Dansk Vet.-T. 1997, 80, 231–234.
Foggie A: Studies on the infectious agent of tickborne fever in sheep. J. Path. Bact. 1951, 63, 1–15.
Foggie A, Hood CS: Adaptation of the infectious agent of tick-borne fever to guinea-pigs and mice. J. comp. Path. 1961, 71, 414–427.
Greig B, Asanovich KM, Armstrong PJ, Dumler JS: Geographic, clinical, serologic, and molecular evidence of granulocytic ehrlichiosis, a likely zoonotic disease, in Minnesota and Wisconsin dogs. J. clin. Microbiol. 1996, 34, 44–48.
Gribble DH: Equine ehrlichiosis. J. Amer. vet. med. Ass. 1969, 155, 462–469.
Hermann M, Baumann D, Lutz H, Wild P: Erster diagnostizierter Fall von equiner Ehrlichiosis in der Schweiz. (The first diagnosed case of equine ehrlichiosis in Switzerland). Pferdeheilkd. 1985, 1, 247–250.
Hudson JR: The recognition of tick-borne fever as a disease of cattle. Brit. vet. J. 1950, 106, 3–17.
Johansson K-E, Pettersson B, Uhlén M, Gunnarsson A, Malmquist M, Olsson E: Identification of the causative agent of granulocytic ehrlichiosis in Swedish dogs and horses by direct solid phase sequencing of PCR products from the 16S rRNA gene. Res. vet. Sci. 1995, 58, 109–112.
KorbutiakE, Schneiders DH: First confirmed case of equine ehrlichiosis in Great Britain. Equine vet. Educ. 1994, 6, 303–304.
Lewis GE, Huxsoll DL, Ristic M, Johnson AJ: Experimentally induced infection of dogs, cats and nonhuman primates with Ehrlichia equi, etiologic agent of equine ehrlichiosis. Amer. J. vet. Res. 1975, 36, 85–88.
MacLeod J: Preliminary studies in the tick transmission of louping ill. II. A study of the reaction of sheep to tick infestation. Vet. J. 1932, 88, 216–284.
MacLeod J, Gordon WS: Studies in tick-borne fever of sheep. 1. Transmission by the tick, Ixodes ricinus, with a description of the disease produced. Parasitology 1933, 25, 273–283.
Madigan JE, Hietala S, Chalmers S, De Rock E: Seroepidemiologic survey of antibodies to Ehrlichia equi in horses of northern California. J. Amer. vet. med. Ass. 1990, 196, 1962–1964.
Madigan JE: Equine ehrlichiosis. In: Woldehiwet Z, Ristic M (eds.): Rickettsial and chlamydial diseases of domestic animals. Pergamon Press, Oxford, 1993, 209–214.
Madigan JE, Richter PJ, Kimsey RB, Barlough JE, Bakken JS, Dumler JS: Transmission and passage in horses of the agent of human granulocytic ehrlichiosis. J. infect. Dis. 1995, 172, 1141–1144.
Madigan JE, Barlough JE, Dumler JS, Schankman NS, De Rock E: Equine granulocytic ehrlichiosis in Connecticut caused by an agent resembling the human granulocytotropic ehrlichia. J. clin. Microbiol. 1996, 34, 434–435.
Nicholson WL, Comer JA, Sumner JW, Gingrich-Baker C, Coughlin RT, Magnarelli LA, Olson JG, Childs JE: An indirect immunofluorescence assay using a cell culture-derived antigen for detection of antibodies to the agent of human granulocytic ehrlichiosis. J. clin. Microbiol. 1997, 35, 1510–1516.
Olsson Engvall E, Pettersson B, Persson M, Artursson K, Johansson K-E: A 16S rRNA-based PCR assay for detection and identification of granulocytic Ehrlichia species in dogs, horses and cattle. J. clin. Microbiol. 1996, 34, 2170–2174.
Rikihisa Y: The tribe Ehrlichieae and ehrlichial diseases. Clin. Microbiol. Rev. 1991, 4, 286–308.
Scott GR: Annual report, Centre of Tropical Veterinary Medicine, Edinburgh, 1978, 20.
Scott GR: Annual report, Centre of Tropical Veterinary Medicine, Edinburgh, 1984, 21.
Stannard AA, Gribble DH, Smith RS: Equine ehrlichiosis: A disease with similarities to tick-borne fever and bovine petechial fever. Vet. Rec. 1969, 84, 149–150.
Stuen S, Hardeng F, Larsen HJ: Resistance to tickborne fever in young lambs. Res. vet. Sci. 1992, 52, 211–216.
Stuen S, Gmnstol H, Larsen HJS: Experimental inoculation of horses with Ehrlichia phagocytophila. In Proceedings: The Second International Conference on Tick-borne Pathogens at the Host-Vector Interface. Kruger National Park, South Africa 1995, 240–243.
Stuen S: Experimental tick-borne fever infection in reindeer (Rangifer tarandus tarandus). Vet. Rec. 1996, 138, 595–596.
Stuen S, Olsson Engvall E, Artursson K: The persistence of Ehrlichia phagocytophila infection in lambs in relation to clinical parameters and antibody responses. Vet. Rec. 1998, 143. In press.
Sumner JW, Nicholson WL, Massung RF: PCR amplification and comparison of nucleotide sequences from the groESL heat shock Operon of Ehrlichia species. J. clin. Microbiol. 1997, 35, 2087–2092.
Telford SR, III, Dawson JE, Katavolos P, Warner CK, Kolbert CP, Persing DH: Perpetuation of the agent of human granulocytic ehrlichiosis in a deer tick-rodent cycle. Proc. natl. Acad. Sci. USA, 1996, 93, 6209–6214.
Tizard I: Veterinary immunology. 4th edition, WB Saunders Company, Philadelphia, 1992.
Tuomi J: Experimental studies on bovine tick-borne fever. 2. Differences in virulence of strains in cattle and sheep. Acta path, microbiol. scand. 1967a, 70, 577–589.
Tuomi J: Experimental studies on bovine tick-borne fever. 3. Immunological strain differences. Acta path, microbiol. scand. 1967b, 71, 89–100.
Woldehiwet Z, Scott G: Immunological studies on tick-borne fever in sheep. J. comp. Path. 1982, 92, 457–467.
Wöldehiwet Z, Scott GR: Tick-borne (pasture) fever. In: Woldehiwet Z., Ristic M. (eds.): Rickettsial and chlamydial diseases of domestic animals. Pergamon Press, Oxford, 1993: 233–254.
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The authors want to thank Ulla-Britt Wikstrøm for excellent technical assistance.
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Stuen, S., Artursson, K. & Engvall, E.O. Experimental Infection of Lambs with an Equine Granulocytic Ehrlichia Species Resembling the Agent that Causes Human Granulocytic Ehrlichiosis (HGE). Acta Vet Scand 39, 491–497 (1998). https://doi.org/10.1186/BF03547775
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DOI: https://doi.org/10.1186/BF03547775