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The Prevalence of Mycoplasma hyopneumoniae in Pig Herds in Western Finland Based on the Demonstration of Antibodies in Colostrum by ELISA


Swine enzootic pneumonia causes heavy economical losses in fattening herds. The aim of this study was to get an estimate of the prevalence of Mycoplasma hyopneumoniae in the province of Vaasa in Western Finland. There were 112 farrowing herds randomly chosen for a survey. Farmers were asked to milk colostrum samples from every sow. Owners of 22 herds did not send enough samples for a herd diagnosis. Altogether 1773 samples (mean 18.1 samples/herd) were analysed in an indirect ELISA for antibodies to Mycoplasma hyopneumoniae. Thirty-five herds were concluded to be infected with Mycoplasma hyopneumoniae. In these herds the mean prevalence of samples with antibodies was 16.3% (range 2.3%-70.0%, median 8.6%). The infected herds were significantly larger than the rest of the herds (no. of sows 27.0 vs. 18.4 respectively). The true prevalence of Mycoplasma hyopneumoniae infec-tion in the province of Vaasa was estimated to be somewhat lower than the apparent prevalence of 39%. The application of colostrum serology is discussed.


  • Barfod K: Sundhedskontrol via râmaelksprover–en overvejelse vaerd. (Health control by colostrum-a method under consideration). Hyologisk Tidsskrift Svinet 1990, no.10, 35–37.

    Google Scholar 

  • Bommeli WR, Nicolet JA: A method for the evaluation of the enzyme-linked immunoassay results for diagnosing enzootic pneumonia in pig herds. Proceedings Third International Symposium of the World Association of Veterinary Laboratory Diagnosticians 1983, 2, 439–442.

    Google Scholar 

  • Bouwkamp FT, Stegeman JA, Kimman TG: Use of colostrum to detect antibodies against glycoprotein I of Aujeszky’s disease virus. Vet. Ree. 1993, 133, 591–593.

    CAS  Google Scholar 

  • Eberli A: Evaluation eines ELISA zum Nachweis von Antikörpern gegen Mycoplasma hyopneumoniae im Blut und in der Milch von Schweinen. (Evaluation of an ELISA for the detection of antibodies to Mycoplasma hyopneumoniae in serum and colostrum of swine). Thesis. Universität Zürich, 1987. 48 pp.

    Google Scholar 

  • Feld NC, Qvist P, Ahrens P, Friis NF, Meyling A: A monoclonal blocking ELISA detecting serum antibodies to Mycoplasma hyopneumoniae. Vet. Microb. 1992, 30, 35–46.

    CAS  Article  Google Scholar 

  • LePotier MF, Abiven P, Kobish M: A blocking ELISA using a monoclonal antibody for the serological detection of Mycoplasma hyopneumoniae. Res. Vet. Sci. 1994, 55, 338–345.

    Article  Google Scholar 

  • Levonen K: Ternimaitotutkimus: tavoitteet ja tähänastiset tulokset. (Colostrum serology: goals and results so far). Sika 1990, no. 2, 14–15.

    Google Scholar 

  • Levonen K: Detection of enzootic pneumonia in pig herds using an enzymelinked immunosorbent assay in sow colostrum. Res. Vet. Sei. 1994a, 56, 111–113.

    CAS  Article  Google Scholar 

  • Levonen K, Veijalainen P, Seppänen J: Actinobacillus pleuropneumoniae serotype 2 antibodies in sow colostrum in Finnish pig-health-scheme herds. J. of Vet. Med. 1994b, series B 41, 567–573.

    Google Scholar 

  • Martin SW, Shoukri M, Thorburn MA: Evaluating the health status of herds based on tests applied to individuals. Prev. Vet. Med. 1992, 14, 33–43.

    Article  Google Scholar 

  • Mori Y, Hamaoka T, Sato S: Use of monoclonal antibody in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against Mycoplasma hyopneumoniae. Isr. J. Med. Sci. 1987, 23, 657–662.

    CAS  PubMed  Google Scholar 

  • Morris CR, Gardner IA, Hietala SK, Carpenter TE, Anderson RJ, Parker KM: Persistence of passively acquired antibodies to Mycoplasma hyop-neumoniae in a swine herd. Prev. Vet. Med. 1994, 21, 29–41.

    Article  Google Scholar 

  • Rautiainen E, Hautala M, Saranpää T, Virtala A-M: A comparative study of feeder pig units using dry or liquid feeding in the western part of Finland. Part 1: The mortality rate, the causes of death and the gastric ulceration. Suomen eläinlääkäril. 1991, 97, 284–296. (English abstract.)

    Google Scholar 

  • Rautiainen E, Nyberg M, Levonen K: The use of sow colostrum samples for regular herd health control of Mycoplasma hyopneumoniae infection. Proceedings of the 14th IPVS congress, Bologna, Italy, July 7-10, 1996. p. 219.

    Google Scholar 

  • Ross RF: Mycoplasmal diseases. In: Leman {etet al.} (ed.): Diseases of swine. Iowa State University Press, Ames 1992, 537–551.

    Google Scholar 

  • Sorensen V, Barfod K, Feld NC, Vraa-Andersen L: Application of enzyme-linked immunosorbent assay for the surveillance of Mycoplasma hyo-pneumoniae infection in pigs. Rev. sci. tech. Off. int. Epiz. 1993, 12, 593–604.

    CAS  Article  Google Scholar 

  • Tuovinen VK, Grohn YT, Straw BE: Health classification of multisource feeder pigs–a field trial. Prev. Vet. Med. 1994, 20, 11–22.

    Article  Google Scholar 

  • Tuovinen VK, Heinonen ML, Suutari EL: Health matching of feeder pigs - good results. Proceedings of the 14th IPVS congress, Bologna, Italy, July 7-10, 1996. p. 548.

    Google Scholar 

  • Volmer R, Thure S, Sperling H, Frost JW: Pig herd control and veterinary services–serological examination of the colostrum of sows. Tierärztliche Umschau 1994, 49, 421–426.

    Google Scholar 

  • Wallgren P, Bölske G, Gustafsson S, Mattsson S, Fossum C: Humoral immune response to Mycoplasma hyopneumoniae in sows and offsprings following an outbreak of mycoplasmosis. Vet. Microb. 1998, 60, 193–205.

    CAS  Article  Google Scholar 

  • Yagihashi T, Kazama S, Tajima M: Seroepidemiology of mycoplasmal pneumonia of swine in Japan as surveyd by an enzyme-linked immunosorbent assay. Vet. Microb. 1993, 34, 155–166.

    CAS  Article  Google Scholar 

  • Zimmermann W, Tschudi P, Nicolet J: ELISA-Serologie in Blut und Kolostralmilch: eine Möglichkeit zur Überwachung der enzootischen Pneumoniae (EP) in Schweine-Beständen. (ELISA serology on milk and serum: a possibility to a herd health control of enzootic pneumonia in swine herds.) Schweiz. Arch. Tierheilk. 1986, 128, 299–306.

    CAS  Google Scholar 

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This study was supported by the provincial govern-ment of Vaasa and by the local slaughterhouses Itikka, Pohjanmaan Liha, Pouttu, Snellman and Saar-ioinen. The author wants to thank Veikko Tuovinen, Pirjo Veijalainen, Anna-Maija Virtala and Per Wallgren for valuable comments.

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Rautiainen, E. The Prevalence of Mycoplasma hyopneumoniae in Pig Herds in Western Finland Based on the Demonstration of Antibodies in Colostrum by ELISA. Acta Vet Scand 39, 325–330 (1998).

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  • swine enzootic pneumonia
  • survey
  • carrier
  • bias
  • sensitivity
  • specificity
  • positive pre-dictive value