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Extraction and Characterization of Native Canine Bone Morphogenetic Protein (cBMP) Qualified with Osteoinductive

Extrahering och karakterisering av nativt morfogenetiskt protein (cBMP) från hundben med verifierad osteoinduktivitet

Acta Veterinaria Scandinavica volume 39, pages 165–171 (1998)Cite this article

Abstract

Bone morphogenetic protein (BMP) was extracted from canine bone matrix, partially purifed and tested for osteoinductivity. A radiographically and histologically detectable ectopic bone formation was induced by 6.0 mg canine (cBMP) in muscle pouch of BALB mouse at 21 days post implantation. Characterization of the cBMP preparation by a gel filtration chromatography defined that the material consisted of proteins or protein complexes with molecular weights from 4 to 120 kD. Isoelectric focusing showed that the molecules were acidic with isoelectric points of 4.6–5.6.

Sammanfattning

Benmorfogenetiskt protein (cBMP) extraherades från hundben, renades partiellt och testades för osteoinduktivitet. En röngenologiskt och histologiskt påvisbar ektopisk benybildning inducerades med 6 mg hund (c) BMP i muskulaturen hos BALB möss 21 dagar efter implantationen. Karakteriseringen av cBMP preparatet med gelflltrations- kromatografi visade att preparatet bestod av proteiner eller proteinkomplex med molekylvikter mellan 4 till 120 kD. Isoelektrisk focusering visade att molekylerna var sura och hade isoelektriska punkter från 4.6-5.6.

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Authors and Affiliations

  1. Surgical Bioimplants Center, Institute of Medical Technology, University of Tampere, P.O. Box 607, FIN-33101, Tampere, Finland

    J. Oksanen MD, A. Marttinen & T.S. Lindholm

  2. Department of Clinical Sciences Faculty of Veterinary Medicine, University of Helsinki, Finland

    S. Paatsama

Authors
  1. J. Oksanen MD
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  2. A. Marttinen
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  3. S. Paatsama
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  4. T.S. Lindholm
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Correspondence to J. Oksanen MD.

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Oksanen, J., Marttinen, A., Paatsama, S. et al. Extraction and Characterization of Native Canine Bone Morphogenetic Protein (cBMP) Qualified with Osteoinductive. Acta Vet Scand 39, 165–171 (1998). https://doi.org/10.1186/BF03547789

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Acta Veterinaria Scandinavica

ISSN: 1751-0147