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The Effect of Prednisolone on Canine Neutrophil Function: In Vivo and in Vitro Studies

In vivo- och in vitroeffekter av prednisolon på granulocytfunktioner hos hund

Acta Veterinaria Scandinavica volume 39, pages 201–213 (1998)Cite this article

Abstract

The in vivo effect of a therapeutic dose of prednisolone on canine neutrophil adherence, random migration, Chemotaxis, phagocytosis of IgG and C3b opsonized yeast cells, chemiluminescence, Fc- and CR3-receptor expression was investigated. Prednisolone was also added in vitro to neutrophils as isolated cells and in whole blood. In the in vivo study, prednisolone increased the IgG mediated ingestion of yeast cells and the number of activated neutrophils in the phagocytosis assay, while flow cytometric investigation of the IgG-receptor FcγRIII with a monoclonal antibody showed similar expression before, during and after treatment. Prednisolone also increased the ingestion of C3b-opsonized yeast cells, while the expression of CR3-receptors (CD11b CD18) measured by flow cytometry was unchanged. Chemiluminescence and the chemotactic response towards zymosan activated serum were increased, while adherence to nylon wool was decreased. The in vitro studies revealed that prednisolone had no or a dampening effect on neutrophils in cell suspensions. Adherence as well as IgG mediated ingestion was decreased at the highest prednisolone concentration (800 ng/ml) in whole blood. The present study suggests that the part of the antiinflammatory effect of corticosteroids mediated through their influence on neutrophils, besides reduced adherence, may be exerted by increased clearance of microorganisms and IgG-complexes through an elevated functional capacity.

Sammendrag

I föreliggande studie undersöktes in vivo-effekten av en terapeutisk dos prednisolon på neutrofilers adhesion, migration/kemotaxis, fagocytos och chemiluminescence hos hund. Prednisolonets påverkan på antalet FcγIII-receptorn och CR3-receptorer studerades också. Prednisolon tillsattes även in vitro till neutrofilsuspension och effekten på neutrofilernas funktion studerades. Resultaten visar att det föreligger skillnader mellan effekten av prednisolon in vivo och in vitro. När prednisolon gavs in vivo, minskade neutrofilernas adhesion och den fagocyterande och avdödande förmågan ökade. Antalet Fc- och CR3-receptorer påverkades inte av behandlingen. När prednisolonet tillsattes in vitro till cellsuspensioner hade det ingen eller dämpande effekt på neutrofilerna.

Resultaten tyder på att en del av den antiinflammatoriska effekten vid kortisonterapi in vivo, förutom den minskade adhesiviteten, kan vara ökad eliminering av mikroorganismer och IgG-komplex genom ökad kapacitet hos neutrofilerna.

References

  • Baltch AL, Hammer MC, Smith RP, Bishop MB, Suth-pen NT, Egy MA, Michelsen PB: Comparision of the effect of three adrenal corticosteroids on human granulocyte function against Pseudomonas aeruginosa. The Journal of Trauma, 1986, 26, 525–533.

    Article  CAS  Google Scholar 

  • Barr RD, Koekebakker M, Milner RA: Hydrocorti-sone-A possible regulator of human granulopoiesis. Scand. J. Hematol. 1983, 31, 31–38.

    Article  CAS  Google Scholar 

  • Clark RAF, Gallin JI, Fauci AS: Effects of in vivo prednisone on in vitro eosinophil and neutrophil adherence and Chemotaxis. Blood. 1979, 53, 4, 633–641.

    Article  CAS  Google Scholar 

  • Colburn WA, Sibley CR, Buller RH: Comparative serum prednisone and Prednisolon concentrations following prednisone or prednisolone administration to beagle dogs. J. Pharm. Sci. 1979, 65, 7, 997–1001.

    Article  Google Scholar 

  • Cupps TR, Fauci AS: Corticosteroid-Mediated im-munoregulation in man. Immunol. Rev. 1982, 65, 133–155.

    Article  CAS  Google Scholar 

  • Feldman EC, Nelson RW: Canine and Feline endocrinology and reproduction. Darlene Pedersen (editor). Saunders Company. 1987,218–227.

  • Forslid J, Hed J: Hydrocortisone both decreases the up-regulation of complement receptors CR1 and CR3 and the ingestion process of human granulocytes. Int. Archs. Allergy appl. Immun. 1987, 84, 345–350.

    Article  CAS  Google Scholar 

  • Guelfi JF, Courdouhji MK, Alvinerie M, Toutain PL: In vivo and in vitro effects of three glucocorticoids on blood leucocyte Chemotaxis in the dog. Vet. Immunol. Immunopathol. 1985, 10, 245–252.

    Article  CAS  Google Scholar 

  • Hed J: The extinction of fluorescence by chrystal violet and its use to differentiate between attached and ingested microorganisms in phagocytosis. FEMS Lett. 1977,1, 357–361.

    Article  Google Scholar 

  • Hirsch GJ, Church AB: Adrenal steroids and infection: the effect of cortisone administration on polymorphonuclear leucocytic functions and on serum opsonins and bactericidins. J. Clin. Invest. 1961,40, 794–798.

    Article  CAS  Google Scholar 

  • Inada K, Ogasawara M, Suzuki M, Kirikae T, Mori KJ, Yoshida M: Enhancement of in-vitro granulopoiesis and induction of differentiation of Ml cells by a glucocorticoid-dependent factor produced from P388D1 cells. Leukemia Research. 1988,12, 305–314.

    Article  CAS  Google Scholar 

  • Kuzniar J, Sajewicz Kopec W, Hruby Z: Chemilumi-nescence of neutrophils in patients with glomeru-linephritis treated with methylprednisolone. International Urology and Nephrology. 1991, 23, 527–534.

    Article  CAS  Google Scholar 

  • Levine PH, Hardin JC, Scoon KL, Krinsky NI: Effect of corticosteroids on the production of superoxide and hydrogen peroxide and the appearance of chemiluminescence by phagocytosing polymorphonuclear leucocytes. Inflammation. 1981, 5, 19–27.

    Article  CAS  Google Scholar 

  • Lewis SL, van Epps DE: Demonstration of specific receptors for fluoresceinated casein on human neutrophils and monocytes using flow cytometri. Inflammation. 1983, 7, 363–375.

    Article  CAS  Google Scholar 

  • Liles WC, Dale DC, Klebanoff SJ: Glucocorticoids inhibit apoptosis of human neutrophils. Blood 1995, 86, 3181–3188.

    Article  CAS  Google Scholar 

  • MacGregor RR, Spagnuolo PJ, Lentnek AL: Inhibition of granulocyte adherence by ethanol, prednisone and aspirin measured with an assay system. N. Engl. J. Med. 1974,291, 642–646.

    Article  CAS  Google Scholar 

  • Mandell GL, Rubin W, Hook EW: The effect of on NADH oxidase inhibitor (hydrocortisone) on polymorphonuclear leucocyte bactericidal activity. J. Clin. Invest. 1970, 49, 1381–1388.

    Article  CAS  Google Scholar 

  • Molina JM, Garrido A, Anguiano A, Uceda A, Serrano E: Experimental study of the effect of 6-methyl-prednisolone on ingestion-microbicidal function activity in dog neutrophils. Comp. Immun. Microbiol. Infect. Dis. Vol. 1991, 14, 21–29.

    Article  CAS  Google Scholar 

  • Morris DD, Strzemienski PJ, Gaulin G, Spencer P: The effect of corticosteroid administration on the migration, phagocytosis and bactericidal capacity of equine neutrophils. Cornell Vet., 1988, 78, 243–252.

    CAS  PubMed  Google Scholar 

  • Motomura S, Katsuno M, Kaneko S, Ibayashi H: The effect of hydrocortisone on the production and differentiation of granulocyte/macrophage progenitor cells in long-term bone marrow cultures. Exp. Hematol. 1983,11, 56–62.

    CAS  PubMed  Google Scholar 

  • Niwa Y, Somiya K, Miyachi Y, Kanoh T and Sakane T. Luminol independent chemiluminescence by phagocytes is markedly enhanced by dexametha-sone, not by other corticosteroids. Inflammation. 1987,11, 163–174.

    Article  CAS  Google Scholar 

  • Olds J W, Reed WP, Eberle B, Kisch AL: Corticosteroids, serum and phagocytosis: In vitro and in vivo studies. Infect, and Immun. 1974, 9, 524–529.

    Article  CAS  Google Scholar 

  • Phillips TR, Yang WC, Schulz RD: The effects of glu-cocorticosteroids on the chemiluminescence response of bovine phagocytic cells. Vet. Immunol, and Immunopathol. 1987,14, 245–256.

    Article  CAS  Google Scholar 

  • Roth JA, Kaeberle ML: Effects of in vivo dexametha-sone administration on in vitro bovine polymorphonuclear leucocyte function. Infection and Immunity. 1981,33, 434–441.

    Article  CAS  Google Scholar 

  • Suda T, Miura Y, Ijima H, Ozawa K, Motoyoshi K, Takuku F: The effect of hydrocortisone on human granulopoeisis in vitro with cytochemical analysis of colonies. Exp. Hematol. 1983,11, 114–121.

    CAS  PubMed  Google Scholar 

  • Trowald-Wigh G, Håkansson L, Johannisson A, Nor-rgren L: Hård af Segerstad, C. Leucocyte adhesion protein deficiency in Irish setter dogs. Veterinary Immunology and Immunopathology. 1991,32, 261–280.

    Google Scholar 

  • Trowald-Wigh G, Thorén-Tolling K: Phagocytosis induced by yeast cells in canine granulocytes: A methodological study. Acta Vet. Scand. 1990a, 31, 87–95.

    CAS  PubMed  Google Scholar 

  • Trowald-Wigh G, Thorén-Tolling K: Chemilumines-cence and Chemotaxis assay of canine granulocytes: A methodological study. Acta Vet Scand. 1990b, 31, 79–86.

    CAS  PubMed  Google Scholar 

  • Webster RO, Hong SR, Johnston RB Jr, Henson PM: Biological effects of the human complement fragments C5a and C5a des Arg on neutrophil function. Immunopharmacology. 1980,2, 201–219.

    Article  CAS  Google Scholar 

  • Wilkinsson PC: Chemotaxis and inflammation. 1974. Churchill Livingstone, London.

    Google Scholar 

  • Zigmond SH, Hirsch JG: Leucocyte locomotion and Chemotaxis. New methods for evaluation and demonstration of a cell derived chemotactic factor. J. Exp. Med. 1973,137, 387–410.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors wish to thank Ulla Nestor for excellent and skillful laboratory assistance. We also wish to thank Inger Sjöstedt for hematological analysis and Åsa Karlsson for serum cortisol/prednisolone determinations. This study was supported by grants from Agria Insurance Company, Stockholm, Sweden. The flow cytometry equipment was financed by the Knut & Alice Wallenberg foundation.

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Authors and Affiliations

  1. Department of Medicine and Surgery, Swedish University of Agricultural Sciences, Box 7018, S-75 007, Uppsala, Sweden

    G. Trowald-Wigh

  2. Department of Clinical Chemistry, University Hospital, Uppsala, Sweden

    L. Håkansson

  3. Department of Pathology, Swedish University of Agricultural Sciences, Uppsala, Sweden

    A. Johannisson

  4. Department of Genetics, Uppsala University, Uppsala, Sweden

    A. Johannisson

  5. National Veterinary Institute, Uppsala, Sweden

    L.E. Edqvist

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  1. G. Trowald-Wigh
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  2. L. Håkansson
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  3. A. Johannisson
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  4. L.E. Edqvist
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Correspondence to G. Trowald-Wigh.

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Trowald-Wigh, G., Håkansson, L., Johannisson, A. et al. The Effect of Prednisolone on Canine Neutrophil Function: In Vivo and in Vitro Studies. Acta Vet Scand 39, 201–213 (1998). https://doi.org/10.1186/BF03547793

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Acta Veterinaria Scandinavica

ISSN: 1751-0147