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The Diagnosis of Pseudorabies by the Fluorescent Antibody Technique
Acta Veterinaria Scandinavica volume 8, pages 360–368 (1967)
Abstract
Fluorescent antibody staining (FA) for pseudorabies has been performed on frozen sections from the cerebrum, cerebellum, and pons from 51 piglets suspected of pseudorabies.
Pseudorabies virus could be demonstrated in the brains of 23 of these pigs both by FA and by rabbit and/or tissue culture inoculation. Most fluorescent cells were found in the cerebral cortex. It is concluded that FA staining may become a reliable alternative to already existing methods for diagnosis of pseudorabies.
References
Albrecht, P., D. Blaskovic, J. Jakubie & J. Lesso: Demonstration of Pseudorabies virus in chick embryo cell cultures and infected animals by the fluorescent antibody technique. Acta virol. 1963, 7, 289–296.
Borgen, H. C. & H. J. Bendixen: Om udbredelsen af pseudorabies-virusinfektioner blandt kvaeg og svin i Danmark. Nord. Vet-Med. 1965, 17, 672–679.
Cherry, W. B., M. Goldman & T. R. Carski: Fluorescent antibody techniques in the diagnosis of communicable diseases. Public Health Service Publication no. 729. United States Government Printing Office, Washington D.C. 1961.
Csontos, L.: Determination of virus content, and possible diagnostic value of organs from pigs died of Aujeszky's disease. Acta vet. Acad. Sci. hung. 1966, 16, 219–222.
Csontos, L. & A. Széky: Gross and microscopic lesions in the nasopharynx of pig with Aujeszky's disease. Acta vet. Acad. Sci. hung. 1966, 16, 175–185.
Flodin, P.: Dextran Gels and their Applications in Gel Filtration. Thesis. Meijels Bokindustri, Halmstad 1962.
Masic, M., M. Excegan & M. Petrovic: Die Bedeutung der Tonsillen für die Pathogenese und Diagnose der Aujeszkyschen Krankheit bei Schweinen. Zbl. Vet.-Med. 1965, (B), 12, 398–405.
McFerran, J. B. & C. Dow: The distribution of the virus of Aujeszky's disease (Pseudorabies virus) in experimentally infected swine. Amer. J. vet. Res. 1965, 26, 631–635.
Pette, J.: Procédés modernes de diagnostic de la Maladie d'Aujeszky. Bull. Off. int. Épiz. 1965, 63, 1835–1851.
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Meyling, A., Bitsch, V. The Diagnosis of Pseudorabies by the Fluorescent Antibody Technique. Acta Vet Scand 8, 360–368 (1967). https://doi.org/10.1186/BF03547822
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DOI: https://doi.org/10.1186/BF03547822