Mycoplasma: Isolation from Prepuce and Semen of Bulls
Acta Veterinaria Scandinavica volume 8, pages 123–135 (1967)
Mycoplasmas were isolated from the prepuce of 12 of 45 bulls and the semen of 2 of the 12 preputial-positive animals, and also from the semen of 1 of the 33 bulls which had not yielded mycoplasmas in the preputial sample. It appears, therefore, necessary to examine both preputial material and semen to detect potential shedders of Mycoplasma. Addition of fresh yeast extract to a solid medium of Bacto PPLO agar + Bacto PPLO serum fraction (1 %) + horse blood (5 %), and 0.005 % of 8 nucleosides (adenosine, cytidine, deoxyadenosine, deoxycytidine, deoxyguanosine, guanosine, thymidine, and uridine) enhanced the number of isolations considerably. Aerobic incubation was preferable to incubation with air plus 10 % CO2.
Albertsen, B. E.: Pleuropneumonia-like organisms in the semen of Danish artificial insemination bulls. Nord. Vet.-Med. 1955, 7, 169–200.
Bakos, K., A. Bane & E. Thai: Incidence of PPLO in the genital tract of cattle. Proc. 16th Int. Vet. Congr., Madrid 1959, 2, 543–545.
Barber, T. L. & J. Fabricant: Primary isolation of Mycoplasma organisms (PPLO) from mammalian sources. J. Bact. 1962, 83, 1268–1273.
Edward, D. G. ff.: An investigation of pleuropneumonia-like organisms from the bovine genital tract. J. gen. Microbiol. 1950, 4, 4–15.
Edward, D. G. ff.: The pleuropneumonia group of organisms: A review together with some new observations. J. gen. Microbiol. 1954, 10, 27–64.
Edward, K. G. ff. & W. A. Fitzgerald: A growth factor needed to isolate organisms of the pleuropneumonia group from the genital tract of cattle. Vet. Rec. 1952, 64, 395–396.
Edward, D. G. ff., J. L. Hancock & S. L. Hignett: Isolation of pleuropneumonia-like organisms from the bovine genital tract. Vet. Rec. 1947, 59, 329–330.
Ernø, H., W. N. Plastridge & M. E. Tourtellotte: Mycoplasma: Nutritional studies of laboratory strains. Acta vet. scand. 1967, 8, 111–122.
Hale, H. H., C. F. Helmboldt, W. N. Plastridge & E. F. Stula: Bovine mastitis caused by a Mycoplasma species. Cornell Vet. 1962, 52, 582–591.
Hartman, H. A., M. E. Tourtellotte, S. W. Nielsen & W. N. Plastridge: Experimental bovine uterine mycoplasmosis. Res. vet. Sci. 1964, 5, 303–310.
Hirth, R. S., W. N. Plastridge & M. E. Tourtellotte: Survival of a Mycoplasma in frozen bull semen. Amer. J. vet. Res. In press.
Hirth, R. S., S. W. Nielsen & W. N. Plastridge: Bovine salpingo-oophoritis produced with semen containing a Mycoplasma. In preparation.
Hirth, R. S., W. N. Plastridge, M. E. Tourtellotte & S. W. Nielsen: Genital mycoplasmosis in cattle and man. J. Amer. vet. med. Ass. 1966, 148, 211–282.
Hoare, M. & D. A. Haig: Isolation of Mycoplasma sp. from the oviducts of dairy cows. Vet. Res. 1964, 76, 956–957.
Karlshøj, K. & N. O. Rasbech: Undersøgelser over spercnielevedygtighed og bakterievœkst i tyresaed fortyndet med forskellige fortyndingsvaesker. Nord. Vet.-Med. 1949, 1, 643–668.
Klieneberger-Nobel, E.: Pleuropneumonia-like Organisms (PPLO): Mycoplasmataceae. Academic Press, Inc., New York 1962.
Nielsen, F.: Sterility in cattle, especially as a result of uterine infection. Proc. 14th Int. Vet. Gongr., London 1949, 3, 105–113.
O'Berry, P. A., F. H. Bryner & A. Frank: Isolation of Mycoplasma from an aborted fetus and vaginal mucus. J. Amer. vet. med. Ass. 1965, 147, 1666.
Speck, J.: PPLO from the genital system of cattle. Mh. Tierheilk. 1962, 14, 244–256. (Original not seen: Vet. Bull. 1963, 33, 130, no. 788).
Taylor-Robinson, D., N. L. Somerson, H. C. Turner & R. M. Chanock: Serological relationships among human Mycoplasma as shown by complement-fixation and gel diffusion. J. Bact. 1963, 85, 1261–1273.
Terpstra, J. I.: A case of “Enzootic Sterility” (Vibrio fetus; pleuropneumonia-like organisms). Proc. 15th Int. Vet. Gongr., Stockholm 1953, 2, 811–816.
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Ernø, H., Plastridge, W.V. & Tourtellotte, M.E. Mycoplasma: Isolation from Prepuce and Semen of Bulls. Acta Vet Scand 8, 123–135 (1967). https://doi.org/10.1186/BF03547838