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Fate of Ingested Histamine in Sheep

I. Disappearance from the Rumen


Histamine ingested with the food or given by mouth disappeared much faster from the rumen than an inert marker (polyethylene glycol), whereas the inactivation of histamine by rumen contents was slow in vitro. This indicated that histamine was absorbed unchanged from the rumen. But when G14-histamine was administered in the same way together with carrier, the biological activity disappeared more rapidly from the rumen than the radioactivity, thus indicating transformation of histamine to inactive metabolites. This study gave no clue as to the reason for the apparent discrepancy between the in vitro and the in vivo experiments.

The in vitro inactivation of histamine in rumen contents was related to the time of feeding, the inactivation in rumen contents aspirated just after feeding being considerably slower than in those aspirated about 3 hrs. after feeding. By inactivation of histamine by rumen contents, formation of small amounts of conjugated histamine was demonstrated both in vivo and in vitro. In vivo experiments indicated that conjugated histamine was absorbed from rumen.

A small flux of radioactivity into the rumen was demonstrated subsequent to subcutaneous injection of C14-histamine.


  • Anrep, G. V., M. S. Ayadi, G. S. Barsoum, J. R. Smith & M. M. Talaat: The excretion of histamine in urine. J. Physiol. (Lond.) 1944, 103, 155–174.

    CAS  Article  Google Scholar 

  • Bergstrøm, S. & G. Hansson: The use of Amberlite IRC-50 for the purification of adrenaline and histamine. Acta physiol. scand. 1951, 22, 87–92.

    Article  Google Scholar 

  • Bain, J. A., A. L. Neal & R. W. Dougherty: The occurrence of histamine and tyramine in rumen ingesta of experimentally over-fed sheep. J. Animal Sci. 1955, 14, 930–935.

    Article  Google Scholar 

  • Dougherty, R. W. & R. M. Cello: Study of toxic factors in the ingesta of cows and sheep. Cornell Vet. 1949, 39, 403–413.

    Google Scholar 

  • Dunér, H. & B. Pernow: Urinary excretion of histamine in healthy human subjects. Scand. J. clin. Lab. Invest. 1956, 8, 296–303.

    Article  Google Scholar 

  • Fowler, H. D.: Histamine in grasses and clovers. Nature (Lond.) 1962, 193, 582–583.

    CAS  Article  Google Scholar 

  • Hydén, S.: A turbidimetric method for the determination of higher polyethylene glycols in biological materials. Ann. roy. agrie. Coll. Sweden 1956, 22, 139–145.

    Google Scholar 

  • Krogh, N.: Studies on alterations in the rumen fluid of sheep, especially concerning the microbial composition, when readily available carbohydrates are added to the food. 1. Sucrose. Acta vet. scand. 1959, 1, 74–97.

    Google Scholar 

  • Macpherson, H. T.: Histamine, tryptamine and tyramine in grass silage. J. Sci. Food Agrie. 1962, 13, 29–32.

    CAS  Article  Google Scholar 

  • Sanford, J.: Formation of histamine in ruminai fluid. Nature (Lond.) 1963, 199, 129–130.

    Article  Google Scholar 

  • Shinozaki, K.: Studies on experimental bloat in ruminants. 4. Identification of amines from rumen ingesta and passage of histamine across the rumen epithelium. Tôhoku J. agrie. Res. 1957, 8, 149–154.

    Google Scholar 

  • Sjaastad, Ø. V.: Determination and occurrence of histamine in rumen liquor of sheep. Acta vet. scand. 1967, 8, 50–70.

    CAS  Google Scholar 

  • Sperber, I., S. Hydén & J. Ekman: The use of polyethylene glycol as a reference substance in the study of ruminant digestion. Ann. roy. agrie. Coll. Sweden 1953, 20, 337–344.

    CAS  Google Scholar 

  • Storniorken, H. & Ø. V. Sjaastad: Diet and histamine in the ruminant. Occurrence of histamine in silage. Arch. int. Pharmacodyn. 1962, 140, 336–340.

    Google Scholar 

  • Tabor, H. & E. Mosettig: Isolation of acetylhistamine from urine following oral administration of histamine. J. biol. Chem. 1949, 180, 703–706.

    CAS  PubMed  Google Scholar 

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Sjaastad, Ø.V. Fate of Ingested Histamine in Sheep. Acta Vet Scand 8, 157–175 (1967).

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