Skip to main content
Download PDF
  • Published:

Determination and Occurrence of Histamine in Rumen Liquor of Sheep

Acta Veterinaria Scandinavica volume 8, pages 50–70 (1967)Cite this article

Abstract

Methods for the determination of histamine in rumen liquor and different feeds are described. The procedures involve adsorption of histamine on Amberlite IRC-50 columns and biological determination of the histamine content of eluates from the ion-exchange resin. By these methods 70—80 % of added histamine were recovered and histamine concentrations as low as 0.01 μg histamine diphosphate per ml of rumen liquor could usually be estimated.

Occurrence and formation of histamine in rumen contents of sheep have been examined with the following results:

  1. 1.

    Feeding invariably increased the concentration of histamine in the rumen, the increments being related to the histamine content of the feed.

  2. 2.

    A small formation of histamine seemed to take place in rumen content both in vivo and in vitro after feeding hay or concentrates.

  3. 3.

    The in vitro formation of histamine in rumen contents was greatly increased when L-histidine (10 mg/ml) was added. The formation of histamine varied widely from day to day and was further related to the time of feeding.

  4. 4.

    Pyridoxal-5-phosphate increased the in vitro formation of histamine in rumen content.

References

  • Ames, B. N. & H. K. Mitchell: Paper chromatography of imidazoles. J. Amer. ehem. Soc. 1952, 74, 252–253.

    Article  CAS  Google Scholar 

  • Barsoum, G. S. & J. Y. Gaddum: The pharmacological estimation of adenosine and histamine in blood. J. Physiol. (Lond.) 1935, 85, 1–14.

    Article  CAS  Google Scholar 

  • Bergstrøm, S. & G. Hansson: The use of Amberlite IRG-50 for the purification of adrenaline and histamine. Acta physiol. scand. 1951, 22, 87–92.

    Article  Google Scholar 

  • Block, R. J. & D. Boiling: The Amino Acid Composition of Proteins and Foods. Charles C. Thomas, Springfield, Illinois, 1951, 576 pp.

    Google Scholar 

  • Bryant, M. P. & I. M. Robinson: An improved nonselective culture medium for ruminai bacteria and its use in determining diural variation in numbers of bacteria in the rumen. J. Dairy Sci. 1961, 44, 1446–1456.

    Article  CAS  Google Scholar 

  • Dain, J. A., A. L. Neal & R. W. Dougherty: The occurrence of histamine and tyramine in rumen ingesta of experimentally over-fed sheep. J. Animal Sci. 1955, 14, 930–935.

    Article  CAS  Google Scholar 

  • Dougherty, R. W.: Analyses of gas and ingesta of bloated cattle. Amer. J. vet. Res. 1942, 3, 401–402.

    Google Scholar 

  • Dunér, H. & B. Pernow: Urinary excretion of histamine in healthy human subjects. Scand. J. clin. Lab. Invest. 1956, 8, 296–303.

    Article  Google Scholar 

  • Florkin, M. & H. S. Mason: Comparative Biochemistry. Academic Press 1963, vol 5, p. 29.

    Google Scholar 

  • Fowler, H. D.: Histamine in grasses and clovers. Nature (Lond.) 1962, 193, 582–583.

    Article  CAS  Google Scholar 

  • Gale, E.: The bacterial amino acid decarboxylases. In, F. F. Nord (ed.) Advances in Enzymology. Interscience Publ., New York. En-zymol. 1946, 6, 1–32.

    CAS  Google Scholar 

  • van der Horst, C. J. G.: Keto-acids and amines in rumen liquid of cattle. Nature (Lond.) 1961, 191, 73–74.

    Article  CAS  Google Scholar 

  • Hydén, S.: A turbidimetric method for the determination of higher polyethylene glycols in biological materials. Ann. Roy. Agric. Coll. Sweden 1956, 22, 139–145.

    Google Scholar 

  • Krogh, N.: Studies on alterations in the rumen fluid of sheep, especially concerning the microbial composition, when readily available carbohydrates are added to the food. 1. Sucrose. Acta vet. scand. 1959, 1, 74–97.

    Google Scholar 

  • Lewis, D.: Amino-acid metabolism in the rumen of the sheep. Brit. J. Nutr. 1955, 9, 215–230.

    Article  CAS  Google Scholar 

  • Lewis, D. & R. S. Emery: Relative deamination rates of amino acids by rumen microorganisms. J. Dairy Sci. 1962, 45, 765–768.

    Article  CAS  Google Scholar 

  • Macpherson, H. T.: Histamine, tryptamine and tyromine in grass silage. J. Sci. Food Agric. 1962, 13, 29–32.

    Article  CAS  Google Scholar 

  • Moir, R. J. & M. Somers: Ruminai flora studies. VIII. The influence of rate and method of feeding a ration upon its digestibility, upon ruminai function, and upon the ruminai population. Aust. J. agric. Res. 1957, 8, 253–265.

    Article  CAS  Google Scholar 

  • Rodwell, A. W.: The occurrence and distribution of aminoacid decarboxylases within the genus lactobacillus. J. gen. Microbiol. 1953, 8, 224–232.

    Article  CAS  Google Scholar 

  • Sanford, J.: Formation of histamine in ruminai fluid. Nature (Lond.) 1963, 199, 129–130.

    Article  Google Scholar 

  • Shinozaki, K.: Studies on experimental bloat in ruminants. 4. Identification of amines from rumen ingesta and passage of histamine across the rumen epithelium. Tôhoku J. agric. Res. 1957, 8, 149–154.

    Google Scholar 

  • Sjaastad, Ø. V. & H. Stormorken: Diet and histamine in the ruminant. Nature (Lond.) 1963, 197, 907–908.

    Article  CAS  Google Scholar 

  • Stormorken, H. & Ø. V. Sjaastad: Diet and histamine in the ruminant. Occurrence of histamine in silage. Arch. int. Pharmacodyn. 1962, 140, 336–340.

    CAS  PubMed  Google Scholar 

  • Thorsell, W.: A qualitative chemical method for the demonstration of “Pauly-positive” bases in rumen contents. Acta chem. scand. 1961, 15, 937–938.

    Article  CAS  Google Scholar 

  • Werle, E. & A. Raub: Über Vorkommen, Bildung und Abbau biogener Amine bei Pflanzen unter besondere Berücksichtigung des Histamins. Biochem. Z. 1948, 318, 538–553.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiology, Veterinary College of Norway, Oslo, Norway

    Ø. V. Sjaastad

Authors
  1. Ø. V. Sjaastad
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sjaastad, Ø.V. Determination and Occurrence of Histamine in Rumen Liquor of Sheep. Acta Vet Scand 8, 50–70 (1967). https://doi.org/10.1186/BF03547852

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03547852

Acta Veterinaria Scandinavica

ISSN: 1751-0147