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Studies on Aspergillus Fumigatus; Stability of Haemolysin and Toxin in Crude Filtrate
Acta Veterinaria Scandinavica volume 9, pages 350–363 (1968)
Summary
The haemolysin and toxin in mycelial filtrates of Aspergillus fumigatus showed a distinct difference in stability when the filtrate was heated at 60°C and pH 6.7, as well as at 40 °C and pH 9.5, the toxin being more stable. When the filtrate was heated at 40 °C and pH 2.5, a rapid inactivation of both haemolysin and toxin occurred.
No haemolytic or toxic activity could be demonstrated in a filtrate with a 6 × 10−2M concentration of formalin after incubation at 37 °C for 3 and 4 days, respectively. Serum prepared with formalin-treated filtrate as antigen had a haemolysin- and toxin-neutralizing effect.
When mercury chloride or p-chloromercuribenzoate in a final concentration of 10−3M was added to the filtrate, immediate blocking of the haemolytic activity, but not of the toxic, was obtained.
Haemolysin inactivated by mercury in a final concentration of 10−3M was reactivated under the influence of L-cysteine or glutathione in a final concentration of 10−2M.
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Rutqvist, L. Studies on Aspergillus Fumigatus; Stability of Haemolysin and Toxin in Crude Filtrate. Acta Vet Scand 9, 350–363 (1968). https://doi.org/10.1186/BF03547862
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DOI: https://doi.org/10.1186/BF03547862