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Preparation of Antigen for the Counterimmunoelectrophoretic Test for Plasmacytosis in Mink*

Acta Veterinaria Scandinavica volume 17, pages 395–402 (1976)Cite this article

Abstract

Counterimmunoelectrophoresis as a test method for making the diagnosis of plasmacytosis in mink demands the specific virus antigen. The method for preparation of the antigen according to Cho & Ingram (1972 a, b) with minor modifications is described in details, and results obtained at 62 antigen preparations are presented. In addition an ultrafiltration method is outlined which may be useful as a replacement for ultracentrifugation procedures used in the technique described by Cho & Ingram (1974).

References

  • Bessis M.: Living Blood Cells and their Ultrastructure. Springer-Verlag, Berlin, Heidelberg, New York 1973, p. 91.

  • Cho H. J. & D. G. Ingram: Antigen and antibody in Aleutian disease in mink. I. Precipitation reaction by agar-gel electrophoresis. J. Immunol. 1972a, 108, (555–557).

    CAS  PubMed  Google Scholar 

  • Cho H. J. & D. G. Ingram: Aleutian disease (AD) of mink: Activation of AD antigen and detection by limmunoelectro-osmophoresis. Proc. Canad. Fed. Biol. Soc. 1972b, 15, abstr. 299.

  • Cho H. J. & D. G. Ingram: Isolation, purification and structure of Aleutian disease virus by immunological techniques. Nature New Biol. 1973, 243, (174–176).

    Article  CAS  Google Scholar 

  • Cho H. J. & D. G. Ingram: The antigen and virus of Aleutian disease in mink. J. immunoL Meth. 1974, 4, (217–228).

    Article  CAS  Google Scholar 

  • Herbert W. J.: Passive haemagglutination with special reference to the tanned cell technique. In D. M. Weir, ed.: Handbook of Experimental Immunology. Vol. I. Blackwell Sci. Publ., Oxford, London, Edinburgh, Melbourne 1973, p. 20.6.

    Google Scholar 

  • Larsen A.: Immunological and viral studies of Aleutian disease in mink. Thesis, Univ, of Utah 1969.

    Google Scholar 

  • Malien M. S., E. L. Ugalde, M. B. Balcazar, J. I. Bolivar & S. Meyran: Precipitation of abnormal serums by Lugol’s solution. Amer. J. clin. Path. 1950, 20, (39–47).

    Article  Google Scholar 

  • Notant G. W., E. C. Hahn, N. H. Sarkar & A. J. Kenyon: Characterisation of Aleutian disease antigens. Nature (Lond.). 1976, 261, (56–58).

    Article  Google Scholar 

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Acknowledgments

M. Hansen, Danish Fur Breeders’ Association, is gratefully acknowledged for having provided the infected material and the mink blood samples. The electron micrograph was kindly prepared by B. Bloch, Dept. of Veterinary Virology and Immunology, Royal Veterinary and Agricultural University, Copenhagen. Excellent technical assistance was given by Mrs. Annie Jørgensen and Mrs. Inge Szabo.

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  1. Department of Veterinary Virology and Immunology, Royal Veterinary and Agricultural University, Bulowsvej 13, DK-1870, Copenhagen, Denmark

    E. Brummerstedt

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  1. E. Brummerstedt
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Brummerstedt, E. Preparation of Antigen for the Counterimmunoelectrophoretic Test for Plasmacytosis in Mink*. Acta Vet Scand 17, 395–402 (1976). https://doi.org/10.1186/BF03547894

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Acta Veterinaria Scandinavica

ISSN: 1751-0147