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Toxoplasmosis in Sheep
The Reliability of a Microtiter System in Sabin and Feldman’s Dye Test
Acta Veterinaria Scandinavica volume 17, pages 426–431 (1976)
Abstract
Sera were tested in 2 series both by the conventional technique (dilution in tubes) and by the microtiter system. In 1 series of comparative examinations the tests were performed with the same batch of accessory factor serum and antigen preparation, and in another series the tests were performed at 2 different laboratories. There was a high correlation between the 2 methods, but the titres obtained by the microtiter system were about 1 to 2 twofold dilutions lower than those obtained by the conventional dilution technique. The 95 % confidence limits by the microtiter system were plus and minus 1 twofold dilution.
References
Aagaard K.: Laboratory methods for the diagnosis of congenital toxoplasmosis. In Human Toxoplasmosis, ed. J. C. Slim. Munksgaard, Copenhagen 1960, 206–210.
Beverley J. K. A. & C. P. Beattie: Standardization of the dye test for toxoplasmosis. J. din. Path. 1952, 5, (350–353).
Engelbrecht E.: The different tests for toxoplasmosis: reproducibility, margins of error and diagnostic meaning. In Toxoplasmosis, ed. D. Hentsch. Hans Huber Publishers, Bern 1971, 25–60.
Evans A. S.: Serological techniques. In Serological Epidemiology, ed. J. R. Paul & G. White. Acad. Press, New York and London 1973, 41–54.
Feldman H. A. & G. A. Lamb: A micromodification of the Toxoplasma dye test. J. Parasit. 1966, 52, (415).
Folkers C.: Evaluation of Aagaard’s method on the search for activator sera for the Sabin-Feldman test in toxoplasmosis. Nature (Lond.) 1964, 202, (307).
Kâss E.: Unders0kelser over Toxoplasma og toxoplasmose. (Toxoplasma and toxoplasmosis). Thesis, Oslo 1954.
Lelong M. &. G. Desmonts: L’emploi du microscope à contraste de phase dans la réaction de Sabin-Feldman. (Phase contrast microscopy in Sabin and Feldman’s dye test). G.R. Soc. Biol. (Paris) 1951, 145, (1660–1661).
Palm G., A. Westphal & E. Bimmer: Zur Technik der Titereinstellung heim Toxoplasma-Serofarbtest. (On the technique of titer adjustment for the toxoplasma-sero-dye test). Zbl. Bakt., I. Abt. Orig. 1958, 171, (202–214).
Sabin A. B. & H. A. Feldman: Dyes as microchemical indicators of a new immunity phenomenon affecting a protozoan parasite (Toxoplasma). Science. 1948, 108, (660–663).
Takátsy G.: Use and field of application of a modified microtitrator apparatus. Hungarian Scientific Instruments 1967, No. 10, 10–18.
Te Punga W. A. & M. E. Penrose: Toxoplasmosis: the effect of citrate, calcium and magnesium ions on the non-specific activity in human sera. N.Z. vet. J. 1965, 13, (11–14).
Tonder O.: Personal communication 1968.
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Waldeland, H. Toxoplasmosis in Sheep. Acta Vet Scand 17, 426–431 (1976). https://doi.org/10.1186/BF03547897
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DOI: https://doi.org/10.1186/BF03547897