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An Improved Bacteriological Method for the Detection of Sulfonamide Residues in Food

Acta Veterinaria Scandinavica volume 17, pages 458–464 (1976)Cite this article

Abstract

The addition of trimethoprim to Mueller-Hinton medium was found to improve the sensitivity of test bacteria towards sulfonamides by 20—50 times. Depending on the test bacteria used, the optimal concentrations of trimethoprim added to the medium are 1 μg per ml (Micrococcus luteus), 0.25 μg per ml (Bacillus stearothermophilus var. calidolactis) and 0.1 μg per ml (Bacillus megaterium). Using Micrococcus luteus or Bacillus megaterium a concentration of 0.25 μg sulfanilamide per ml may be detected, and 0.1 μg per ml may be detected when Bacillus stearothermophilus var. calidolactis is used.

References

  • Bushby S. R. M.: Combined antibacterial action in vitro of trimethoprim and sulphonamides. Postgrad, med. J. 1969, 45, (10–18).

    CAS  Google Scholar 

  • Bushby S. R. M. & G. H. Hitchings: Trimethoprim, a sulphonamide potentiator. Brit. J. Pharmacol. 1968, 33, (72–90).

    CAS  PubMed  Google Scholar 

  • Gudding R.: The suitability of some media and peptones for sulfonamide testing. Acta vet. scand. 1974a, 15, (366–380).

    CAS  PubMed  Google Scholar 

  • Gudding R.: Et enkelt medium til pâvisning av restkonsentrasjoner av sulfonamider. (A simple medium for the detection of sulfonamides). 12th Nord. Vet. Congress, Proc. 1974b, 282.

  • Gudding R. & L. Hellesnes: Pâvisning av sulfonamider i melk. (Detection of sulfonamides in milk). Nord. Vet.-Med. 1973, 25, 248–255.

    CAS  PubMed  Google Scholar 

  • Hitchings G. H.: Species differences among dihydrofolate reductases as a basis for chemotherapy. Postgrad, med. J. 1969, 45, (7–10).

    CAS  Google Scholar 

  • Mueller J. H. & J. Hinton: A protein-free medium for primary isolation of the gonococcus and meningococcus. Proc. Soc. exp. Biol. (N.Y.) 1941, 48, (3130–337).

    Article  Google Scholar 

  • Read R. B., Jr., J. G. Bradshaw, A. A. Swartzentruber & A. R. Brazis: Detection of sulfa drugs and antibiotics in milk. Appl. Microbiol. 1971, 21, (806–808).

    Article  CAS  Google Scholar 

  • Yndestad M. & A. Orbeck Sflrheim: Forekomst av veksthemmende stoffer i normale slaktedyr i Norge i 1973/1974. (Occurrence of growth inhibiting substances in healthy slaughter animals in Norway in 1973/1974). Norsk Vet.-T. 1975, 87, (477–479).

    Google Scholar 

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  1. National Veterinary Institute, Postbox 8156, Oslo Dep., Oslo 1, Oslo, Norway

    Roar Gudding

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  1. Roar Gudding
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Gudding, R. An Improved Bacteriological Method for the Detection of Sulfonamide Residues in Food. Acta Vet Scand 17, 458–464 (1976). https://doi.org/10.1186/BF03547901

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Acta Veterinaria Scandinavica

ISSN: 1751-0147