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Heat Stable Nuclease in Mastitic Milk

Abstract

The production of a heat stable nuclease (deoxyribonuclease) by Staphylococcus aureus was first reported by Cunningham et al. (1956). The detection of the enzyme was simplified by Lachica et al. (1971), who developed an agar diffusion test based on the metachromatic properties of toluidine blue.

References

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  • Lachica R. V. F., C. Genigeorgis & P. D. Hoeprich: Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Appl. Microbiol. 1971, 21, (585–587).

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  • Sandvik O.: Studies on casein precipitating enzymes of aerobic and facultatively anaerobic bacteria. Thesis, Veterinary College of Norway, Oslo 1962.

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  • Schalm O. W. & D. O. Noorlander: Experiments and observations leading to development of the California mastitis test. J. Amer, vet. med. Ass. 1957, 130, (199–204).

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Gudding, R. Heat Stable Nuclease in Mastitic Milk. Acta Vet Scand 17, 501–502 (1976). https://doi.org/10.1186/BF03547906

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  • DOI: https://doi.org/10.1186/BF03547906