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The Applicability of Agar-Diffusion Test in Measuring Deoxyribonucleases in Pig Intestinal Content
Anvendelighet av agar-diffusjonstest ved måling av deoxyribonukleaser i tarminnhold hos gris
Acta Veterinaria Scandinavica volume 17, pages 244–254 (1976)
Abstract
The suitability of an agar-diffusion test (ADT) using toluidine blue deoxyribonucleic acid agar (TDA) for measuring DNase activity in pig intestinal contents was investigated. The ADT was compared with a spectrophotometrical method. Distinct metachromatic zones around wells in the DNA-containing agar, into which the intestinal content was applied, indicated DNase activity. The DNase activity was determined semiquantitatively by making serial twofold dilutions of the intestinal content. The spectrophotometrical method was optimal at pH 7.2. The ADT proved to be most sensitive at pH 5.6. The ability of the 2 methods employed to measure low concentrations of DNases was equal. However, the ADT was considered more suitable than the spectrophotometrical method because ADT measured reduced amounts of enzyme. DNase activity was demonstrated throughout the small intestine and in the large intestine. By the zymogram technique, at least 3 different DNases could be demonstrated in the lower parts of the small intestine, 1 of which could be of extrapancreatic origin.
Sammendrag
Det er blitt undersøkt om en agar-diffusjonstest (ADT) kan anvendes til å måle DNase aktiviteten i tarminnhold hos gris. ADT ble sammenlignet med en spektrofotometrisk metode. I agaren som inneholdt DNA, ble det laget brønner som ble fylt med tarminnhold og rundt disse fremkom metakromatiske soner som indikasjon på DNase aktivitet. Ved 2-folds fortynning av tarminnholdet kunne ADT nyttes for semikvantitativ bestemmelse av DNase aktiviteten. ADT var mest sensitiv ved pH 5,6. Ved hjelp av de 2 metoder kunne en påvise om lag like lave konsentrasjoner av DNase. ADT ble likevel ansett for mer hensiktsmessig enn den spektrofotometriske metode fordi det ved ADT ble målt mindre mengder enzym. DNase aktivitet ble påvist i hele tynntarmen samt i tykktarmen. Ved bruk av zymogramteknikk ble det påvist 3 forskjellige DNaser i de nedre deler av tynntarmen hvorav en kan ha annen opprinnelse enn pankreas.
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Liven, E. The Applicability of Agar-Diffusion Test in Measuring Deoxyribonucleases in Pig Intestinal Content. Acta Vet Scand 17, 244–254 (1976). https://doi.org/10.1186/BF03547932
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DOI: https://doi.org/10.1186/BF03547932