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The Effect of Low Temperatures on the Motility of Diphyllobothrium Latum Plerocercoids

Effekten av låga temperaturer på motiliteten av Diphyllobothrium latum plerocercoider

Acta Veterinaria Scandinavica volume 11, pages 236–246 (1970)Cite this article

Abstract

The effect of low temperature exposure on the motility of Diphyl-lobothrium latum plerocercoids was studied, with the particular aim of finding the exposure that immobilizes all the larvae in fish freezing. Both isolated larvae immersed in normal horse serum and larvae enclosed in pieces of muscle tissue of the size of 1 cm3 were tested. The pieces of tissue containing a larva were placed in the middle of a plastic beaker filled with densely packed minced fish flesh. In the central part of this phantom, where the plerocercoids were situated, the temperature decline was considered to take place in the same way as in the interior of a whole fish. A total of 200 isolated larvae were tested, and a temperature of −14° G was found to have a fully immobilizing effect on them. The number of plerocercoids frozen enclosed in muscular tissue was 453, and −10° G was found to immobilize them. The observed difference seems to be mainly due to the cryoprotective properties of serum.

Sammanfattning

Den effekt, som låga temperaturer har på motilitet av Diphyllobothrium latums plerocercoider, undersöktes speciellt med målet att finna den kylbehandling, som immobiliserar alla larver vid nedfrysning av fisk. Både isolerade larver nedsänkta i normalt hästserum samt larver inneslutna i fiskmuskelbitar av 1 cm3 storlek blev testade. Vävnadsbitarna, innehållande en larv, placerades i mitten av en plastbägare fylld med tätt packat finmalet fiskkött. I den centrala delen av denna fantom, där plerocercoiderna var belägna, ansågs temperatursänkningen försiggå på samma sätt, som i de inre delarna av en hel fisk. Inalles 200 isolerade larver undersöktes, och man fann, att temperaturen —14° C immobiliserade dem alla. Antalet plerocercoider nedfrysta i fiskmuskelbitar var 453, och man kunde konstatera, att —10° C hade immobiliserande verkan på dem alla. Den observerade skillnaden beror sannolikt på serumets köldskyddande verkan.

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  1. Department of Food Hygiene, College of Veterinary Medicine, Helsinki, Finland

    K. Salminen

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Salminen, K. The Effect of Low Temperatures on the Motility of Diphyllobothrium Latum Plerocercoids. Acta Vet Scand 11, 236–246 (1970). https://doi.org/10.1186/BF03547984

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Acta Veterinaria Scandinavica

ISSN: 1751-0147