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Distribution of Acid Phosphatase During Autolysis in Bovine Liver

Fördelning av surt fosfatas under autolys i nötlever

Acta Veterinaria Scandinavica volume 11, pages 283–294 (1970)Cite this article

Abstract

The percentage distribution of acid phosphatase between lysosomes and cytoplasm in bovine liver during autolysis at 37°C was investigated. The share of the cytoplasmic acid phosphatase activity of the total acid phosphatase activity in liver tissue increased during autolysis being before incubation 28–42 % and after 24 hrs.' incubation at 37°C 63–94 %. Microbiological contamination increased the proportion of cytoplasmic acid phosphatase.

When bovine liver was incubated at 37°C for 24 hrs., the activity of the total acid phosphatase decreased to about 50 % of the initial activity. During a 16 days' incubation at 4°C the total acid phosphatase activity of bovine liver, however, remained unchanged.

Sammanfattning

Författaren har undersökt hur surt fosfatas, som huvudsakligen är ett lysosomalt enzym, fördelar sig mellan cellens cytoplasma och lysosomer i nötlever under olika stadier av autolys vid en temperatur av 37°C. Det lysosomala sura fosfatasets aktivitet före inkubationen vid 37° var mellan 28 och 42 % av det sura fosfatasets totalaktivitet, och efter 24 timmars inkubation mellan 63 och 94 %. En mikrobiell kontamination ökade under inkubationen det cytoplasmatiska sura fosfatasets relativa andel av totalaktiviteten.

Då nötlever inkuberades vid 37° C under 24 timmar, minskade den totala sura fosfatasets aktivitet till ungefär 50 % av den före inkubationen mätta aktiviteten. Under 16 dagars inkubation vid 4°C förblev däremot nötleverns totala sura fosfatasaktivitet oförändrad.

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  1. Department of Food Hygiene, College of Veterinary Medicine, Helsinki, Finland

    Timo J. Pekkanen

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Pekkanen, T.J. Distribution of Acid Phosphatase During Autolysis in Bovine Liver. Acta Vet Scand 11, 283–294 (1970). https://doi.org/10.1186/BF03547988

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Acta Veterinaria Scandinavica

ISSN: 1751-0147