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Studies of Electrophoresis on Cellulose Acetate Membrane of Serum Proteins from Normal Horses, Sheep and Pigs

Elektroforese-undersøkelser på cellulose-acetat-membran av serumproteiner fra normale hester, sauer og griser

Acta Veterinaria Scandinavica volume 11, pages 295–304 (1970)Cite this article

Abstract

A method for the rapid electrophoresis on a cellulose acetate membrane of serum proteins from horses, sheep and pigs is discussed.

The various main globulin fractions in the serum of these animals were experimentally identified.

Normal values for the percentage composition of serum from normal horses, sheep and pigs were calculated.

In the horse there was great individual variation in the shape of the β-fraction, assumed to be due to different transferrin types. The mean value for β-globulin of 19.5 % in the horse was higher than for the other two species.

The albumin percentage was highest in the sheep and lowest in the pig, 48.5 % and 43.2 % respectively. The sheep had the highest γ-globulin percentage, 22.8 %, while the horse had the lowest with 19.0 %.

Finally the values were compared with corresponding figures reported by other authors and the results discussed.

Sammendrag

Det omtales en metode til hurtigelektroforese på cellulose-acetatmembran av serumproteiner fra hester, sauer og griser.

De enkelte grøvre globulinfraksjoner i disse dyrearters sera ble eksperimentelt identifisert.

Det ble beregnet normalverdier for den prosentvise sammensetning av serum fra normale hester, sauer og griser.

Hos hest var det store individuelle variasjoner i β-fraksjonens form, noe som ble antatt å skyldes forskjellige transferrintyper. Middelverdien for (3-globulin hos hest på 19,5 % var større enn hos de to andre dyrearter.

Albuminprosenten var høyest hos sau og lavest hos gris, henholdsvis 48,5 % og 43,2 %. Sau hadde høyeste γ-globulinprosent, 22,8 %, mens hest hadde den laveste med 19,0 %.

Verdiene ble sluttelig sammenlignet med tilsvarende tall angitt av andre forfattere og resultatene diskutert.

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Acknowledgments

The author wishes to thank Prof. dr. M. Brænd, at present with the Faculty of Veterinary Science, University College, Nairobi, Kenya and Dr. U. Abildgaard, Dept. IX, Ullevål Hospital, Oslo, Norway, for valuable advice and guidance during the experiment. The author owes them both a great debt of gratitude.

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  1. Department of Medicine, Veterinary College of Norway, Oslo, Norway

    Nils Ek

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Ek, N. Studies of Electrophoresis on Cellulose Acetate Membrane of Serum Proteins from Normal Horses, Sheep and Pigs. Acta Vet Scand 11, 295–304 (1970). https://doi.org/10.1186/BF03547989

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Acta Veterinaria Scandinavica

ISSN: 1751-0147