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Urinary Oestrogens in Ovariectomized Cows after Administration of ACTH

Abstract

Two ovariectomized cows were injected intramuscularly once daily with 300 i.u. of ACTH*) on three consecutive days. The urine was collected with the aid of rubber mats. The method for oestrogen analysis comprised acid hydrolysis of the urine, saponification of the phenolic fraction, inethylation and column chromatography followed by fluorimetry (Lunaas 1964 and unpublished).

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Lunaas, T. Urinary Oestrogens in Ovariectomized Cows after Administration of ACTH. Acta Vet Scand 11, 329–330 (1970). https://doi.org/10.1186/BF03547995

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  • DOI: https://doi.org/10.1186/BF03547995