- Published:
The Migration Test on Circulating Bovine Leukocytes and its Possible Application in the Diagnosis of Johne's Disease
Acta Veterinaria Scandinavica volume 11, pages 331–334 (1970)
Abstract
The leukocyte migration test for in vitro studies of delayed type hypersensitivity has recently been reviewed (Søborg & Ben-dixen 1967; Bendixen & Søborg 1969). Søborg & Bendixen applied the test to circulating leukocytes in man and thereby widely increased the potentialities of this test. They obtained high leukocyte yields with only moderate erythrocyte admixture by harvesting the supernatant plasma after sedimentation of the erythrocytes for 60 min. at 37°C in the normal gravitational field (1 × g)- Their procedure was unsuitable for the present investigation because bovine erythrocytes sediment so slowly. Sedimentation after clumping at the interphase of aqueous solutions of polymers, dextran and methylcellulose, in combination with metrizoic acid (Böyum 1968) was tried without success because the vast majority of the leukocytes sedimented together with the erythrocytes. Separation of leukocytes from erythrocytes could not be achieved by differential centrifugation.
References
Bendixen G. & M. Søborg: A leukocyte migration technique for in vitro detection of cellular (delayed type) hypersensitivity in man. A survey. Dan. med. Bull. 1969, 16, 1–6.
Böyum A.: Separation of leukocytes from blood and bone marrow. Scand. J. clin. Lab. Invest. 1968, 21, Suppl. 97, 1–109.
Søborg M. & G. Bendixen: Human lymphocyte migration as a parameter of hypersensitivity. Acta med. scand. 1967, 181, 247–256.
Acknowledgments
The State Veterinary Serum Laboratory, Dr. J. Berg Jørgensen, Dr. H. J. Bendixen and Dr. P. G0rtz, kindly serviced the complement fixation tests and the leukocyte counts. The authors are grateful to Mrs. Kirsten Poulsen for very skilled technical assistance.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hoerlein, A.B., Aalund, O. & Adler, H.C. The Migration Test on Circulating Bovine Leukocytes and its Possible Application in the Diagnosis of Johne's Disease. Acta Vet Scand 11, 331–334 (1970). https://doi.org/10.1186/BF03547996
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1186/BF03547996