Skip to main content
  • Published:

Studies on Parturient Paresis in Dairy Cows. V. On the Composition and Calcium Binding Capacity of Two Bovine Serum Protein Fractions, with Special Regard to Parturient Paresis

Summary

In healthy cows unaffected by imminent or recent calving the protein in serum can directly bind the overwhelming proportion of the bound calcium. In recent calvers this capacity is considerably less. When adding ammonium sulphate to blood serum to 62 % of total saturation a protein fraction precipitates which is mainly albumin. This fraction has a far greater calcium binding capacity than the soluble fraction, which contains most of the serum globulin, and the lowering of this capacity after calving is entirely referable to the former fraction. No difference has been found in these respects between normal cows after calving and cows with parturient paresis.

An analysis of 10 amino acids in the two protein fractions described above showed that the amino acid composition of both exhibits differences between recent calvers and cows outside the calving period, and likewise that each of the two fractions differs in composition between healthy cows after calving and cows with parturient paresis.

References

  • Carlstrom, G.: Studies on parturient paresis in dairy cows. II. Determination of calcium ions in bovine serum. Acta agric. scand. 1955, 5, 357–374.

    CAS  Google Scholar 

  • Carlstrom, G.: Studies on parturient paresis in dairy cows. IV. Calcium and proteins in bovine serum normally, after parturition, and in parturient paresis. Acta vet. scand. 1961a, 2, 330–349.

    CAS  Google Scholar 

  • Carlstrom, G.: Phosphorus, magnesium, and pH in bovine blood serum normally, after parturition, and in parturient paresis. Acta vet. scand. 1961b, 2, 350–356.

    Article  CAS  Google Scholar 

  • Carlstrom, G.: A method for gas chromatographic determination of 11 amino acids in protein hydrolysate. Acta vet. scand. 1968, 9, 71–85.

    Article  CAS  Google Scholar 

  • Farber, L.: Applications of pervaporation. Science 1935, 82, 158.

    Article  CAS  Google Scholar 

  • Fiske, C. H. & Y. Subbarow: The colorimetric determination of phosphorus. J. biol. Chem. 1925, 66, 375–400.

    Article  CAS  Google Scholar 

  • Hallgren, W., G. Carlstrom & G. Jonsson: Studies on parturient paresis in dairy cows. HI. Determination of calcium ion concentration in bovine serum in different hypocalcaemic and pseudohypo- calcaemic conditions. Nord. Vet.-Med. 1959, 11, 217–249.

    CAS  Google Scholar 

  • Hawk, Ph. B., B. L. Oser & W. H. Summerson: Practical Physiological Chemistry, 13th Ed., New York, Toronto, London 1954, p. 630.

    Google Scholar 

  • Kober, P. A.: Pervaporation, perstillation, and percrystallization. J. Amer. chem. Soc. 1917, 39, 944–950.

    Article  CAS  Google Scholar 

  • Michaelis, L.: Der Acetat-Veronal-Puffer. (The acetate-veronal buffer). Biochem. Z. 1931, 234, 139–141.

    CAS  Google Scholar 

  • Reiner, M.: Standard Methods of Clinical Chemistry, Vol. I. New York 1953, p. 88.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Carlström, G. Studies on Parturient Paresis in Dairy Cows. V. On the Composition and Calcium Binding Capacity of Two Bovine Serum Protein Fractions, with Special Regard to Parturient Paresis. Acta Vet Scand 11, 89–102 (1970). https://doi.org/10.1186/BF03548006

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03548006