- Brief Communication
- Published:
Pregnancies from Bovine Oocytes Matured and Fertilized in vitro
Acta Veterinaria Scandinavica volume 30, pages 483–485 (1989)
Abstract
Production of transgenic animals and embryo cloning are only a few examples of new biotechnological methods applied to animal embryos. All these techniques require large amounts of oocytes and early embryos. In many laboratory animals, embryos matured and fertilized in vivo are easily obtained, but with larger domestic species it requires laborious surgical procedures and the number of embryos obtained remains relatively small (Bracken et al. 1982). The in vitro maturation of follicular oocytes derived from slaughterhouse ovaries and their in vitro fertilization provides large numbers of oocytes and embryos with considerably less effort. The final proof of the success in the in vitro maturation and fertilization procedure is the birth of healthy progeny. Also the normal preimplantation development of the embryos gives useful information about the efficiency of the method employed.
References
Bavister BD, Leibfried ML, Liebermann G: Development of preimplantation embryos of the golden hamster in a defined culture medium. Biol. Reprod. 1983, 28, (235–247).
Brackett BG, Bousquet D, Boice ML, Donawick WJ, Evans JF, Dressel MA: Normal development following in vitro fertilization in the cow. Biol. Reprod. 1982, 27, (147–158).
Parrish J J, Susko-Parrish JL, Leibfried-Rutledge ML, Critser ES, Eyestone WH, First NL: Bovine in vitro fertilization with frozen-thawed semen. Theriogenology 1986, 25, (591–600).
Acknowledgments
This study was financially supported by the Ministry of Agriculture and Forestry in Finland. The technical assistance of Mrs. Paula Räty is gratefully acknowledged.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Peura, T., Aalto, J., Rainio, V. et al. Pregnancies from Bovine Oocytes Matured and Fertilized in vitro. Acta Vet Scand 30, 483–485 (1989). https://doi.org/10.1186/BF03548026
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1186/BF03548026