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Corynebacterium Pseudotuberculosis Infection in Goats VIII.

Corynebacterium pseudotuberculosis infeksjon hos geit VIII. Effekten av vaksinasjon mot eksperimentell infeksjon

The Effect of Vaccination against Experimental Infection

Acta Veterinaria Scandinavica volume 30, pages 275–283 (1989)Cite this article

Abstract

The effect of an inactivated vaccine against C. pseudotuberculosis infection was tested on castrated male kids from a herd free from caseous lymphadenitis. The animals were divided into 3 groups with 8 animals in each. Group 1 was immunized with crude filtrated C. pseudotuberculosis toxoid and whole killed organisms, while Group 2 in addition was given levamisole. The kids were vaccinated twice at an interval of 4 weeks. Group 3 consisted of unvaccinated animals. All groups were challenged subcutaneously with live bacteria 4 weeks after the last vaccination.

Unvaccinated animals showed the most severe course of illness after challenge. Development of abscesses in the regional lymph nodes (Inn. subiliaci) was significantly more common in unvaccinated than in vaccinated kids at necropsy 2 months after challenge. There was, however, no such difference between the vaccinated groups, and there was no difference between any of the groups as regards abscess formation at the inoculation site.

In each of the 2 vaccinated groups, there was a titre rise following vaccination in the hemolysis inhibition test, whereas no such rise was seen in the bacterial agglutination test. The titre values in both tests increased significantly after challenge in all the groups, the increase being most rapid in the vaccinated animals.

The present investigation indicates that development of caseous lesions in lymph nodes in goats, following subcutaneous inoculation with C. pseudotuberculosis, can be reduced by an inactivated vaccine containing whole organisms and crude toxin.

Sammendrag

Effekten av en inaktivert vaksine mot eksperimentell infeksjon med C pseudotuberculosis ble undersøkt hos kastrerte bukkekje fra en besetning fri for kascøs lymfadenitt. Dyrene ble delt i 3 grupper med 8 kje i hver. Gruppe 1 ble immunisert med filtrert urenset toksoid av C. pseudotuberculosis og hele drepte bakterier, mens gruppe 2 fikk levamisol i tillegg til dette. Dyrene ble vaksinert 2 ganger med 4 ukers mellomrom. Gruppe 3 bestod av uvaksinerte dyr. Alle 3 grupper ble belastet sub-cutant med levende bakterier 4 uker etter siste vaksinasjon.

Uvaksinerte dyr fikk de mest alvorlige sykdomsforløpene etter belastning. Abscesser i de regionale lymfeknutene (Inn. subiliaci) til inokuleringsstedet ble påvist hos signifikant flere uvaksinerte enn vaksinerte kje ved obduksjon foretatt 2 måneder etter vaksinering. Det var imidlertid ingen forskjell mellom de vaksinerte gruppene i forekomst av dyr med slike abscesser. Abscessdanneise på inokuleringsstedet forekom like vanlig hos dyr i alle grupper.

Etter vaksinering ble det påvist økning i titer ved antihemolysintest i de vaksinerte gruppene. Det ble imidlertid ikke påvist økning i titer ved bakte-rieagglutinasjonstest etter vaksinering. I alle gruppene økte titerverdiene ved begge testene signifikant etter belastning, raskest hos de vaksinerte.

Foreliggende undersøkelse tyder på at en vaksine som inneholder hele drepte bakterier og urenset toksoid, kan redusere dannelsen av abscesser i lymfeknuter etter subcutan inokulering av C. pseudotuberculosis hos geit.

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Acknowledgement

The investigation was supported by grants from the Agricultural Research Council of Norway.

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Authors and Affiliations

  1. Department of Microbiology and Immunology and Department of Pathology, Norwegian College of Veterinary Medicine, P. O. Box 8146, Dep., N-0033 Oslo 1, Oslo, Norway

    Gudmund Holstad, Jon Teige jr. & Hans Jørgen Larsen

  2. State Veterinary Laboratory for Northern Norway, Harstad, Norway

    Gudmund Holstad, Jon Teige jr. & Hans Jørgen Larsen

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  1. Gudmund Holstad
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  2. Jon Teige jr.
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  3. Hans Jørgen Larsen
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Holstad, G., Teige, J. & Larsen, H.J. Corynebacterium Pseudotuberculosis Infection in Goats VIII.. Acta Vet Scand 30, 275–283 (1989). https://doi.org/10.1186/BF03548032

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  • DOI: https://doi.org/10.1186/BF03548032

Keywords

  • whole killed bacteria
  • crude filtrated toxoid
  • levamisole
  • bacterial agglutination test
  • hemolysis inhibition test
  • challenge
  • abscesses

Acta Veterinaria Scandinavica

ISSN: 1751-0147