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Myofibre Changes and Capsule Formation in Mice Infected with Different Strains of Trichinella

Muskeifiberforandringer og kapseidannelse hos mus infisert med fire stammer av Trichinella

Acta Veterinaria Scandinavica volume 30, pages 341–346 (1989)Cite this article

Abstract

Mice were infected with 4 strains of Trichinella with the purpose of describing muscle changes (M. Gastrocnemius, diaphragma) by light and electron microscopy, paying particular attention to capsule formation in a polar bear isolate (T. no. 13).

No differences in myofibre changes or capsule formation were observed between the different strains by light microscopy at 21 and 60 days post infection. Ultra-structural studies of meat samples 60 day post infection with T. no. 13 revealed active capsule formation in muscle fibres, characterized by protrusions and invaginations of the sarcolemma. Basal lamina-like material was located close to the sarcolemma and in a lattice-like pattern in the capsule matrix.

T. no. 13 seems to have a migratory pattern similar to the other strains examined and induces muscle fibre changes undiscernible from those of the 3 other strains. Infected muscle cells are considered to be involved in capsule formation.

Sammendrag

Lysmikropisk undersøkelse av muskelprover (M. gastrocnemius) fra mus 21 og 60 dager p.i. med 3 kjente stammer av Trichinella, samt et isolat fra isbjørn (T. no. 13), viste ingen forskjeller i muskeifiberforandringer eller kapseldannelse.

Elektronmikroskopiske studier av Diaphragma fra mus viste at det fremdeles var aktiv kapseldannelse i muskelceller 60 dager etter infeksjon med T. no. 13. Disse var karakterisert av utvekster og invaginasjoner på sa reol em ma-membranen. Basal-lamina-liknende materiale var lokalisert nær sar-colemma og i et gitterformet mønster i kapsel-matrix.

T. no. 13 synes å ha et felles intracellulært van-dnngsmonster med de andre stammene i denne undersøkelsen og forårsaker muskeIfiber-endringer som ikke kan skilles fra disse. De infiserte muskelcellene deltar i stor grad i kapseldannelsen.

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Authors and Affiliations

  1. The Department of Food Hygiene, Norwegian College of Veterinary Medicine, National Veterinary Institute, P. O. Box 8146, Dep., N-0033 Oslo 1, Oslo, Norway

    Øystein Evensen, Eystein Skjerve & Bjørn Bratberg

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  1. Øystein Evensen
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  2. Eystein Skjerve
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  3. Bjørn Bratberg
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Evensen, Ø., Skjerve, E. & Bratberg, B. Myofibre Changes and Capsule Formation in Mice Infected with Different Strains of Trichinella. Acta Vet Scand 30, 341–346 (1989). https://doi.org/10.1186/BF03548041

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  • DOI: https://doi.org/10.1186/BF03548041

Keywords

  • trichinosis
  • polar strain
  • light microscopy
  • ultrastructure

Acta Veterinaria Scandinavica

ISSN: 1751-0147