- Published:
Improved Method to Estimate Oxygen Consumption, Diffusing Capacity and Blood Flow of Synovial Membrane
Acta Veterinaria Scandinavica volume 30, pages 113–119 (1989)
Abstract
The joint cartilage is depending on the oxygen diffusing from the capillaries of the synovial membrane through the synovial tissue and synovial fluid.
In the present study we describe a new method to calculate the diffusing capacity (DO2), oxygen consumption (VO2) and blood flow of the synovial membrane. The principle of the method is to perfuse the joint cavity with two saline solutions, one with high and one with low oxygen and nitrogen partial pressures and to measure the oxygen and nitrogen partial pressures in the perfusate flowing out of the joint. Using a model of gas exchange between the joint and the membrane a set of equations was derived expressing the relationship between the blood flow, diffusing capacity and oxygen consumption and the oxygen and nitrogen flowing to and from the joint in the two different perfusion situations. In 12 rabbit knee joints we found a blood flow of 0.388 ml/min (SEM 0.027), VO2 of 0.495 µl/min (SEM 0.196) and DO2 of 0.024 µl/min/Torr SEM 0.003 (mean).
References
Bartels H, Harms H: Sauerstoffdissoziationskurven des Blutes von Saugetieren. (Oxygen dissociation curves in mammal blood). Pflügers Arch. 1959, 268, (334–365).
Bünger C: Hemodynamics of the juvenile knee. Acta Orthop. Scand. 1987, 58, Suppl. 222.
Hierton C: Effects of Indometacin, Naproxen and Paracetamol on Regional Blood Flow in Rabbits: A Microsphere Study. Acta pharmacol. ettoxicol. 1981, 49, (327–333).
Lucht U, Bünger C, Krebs B, Hjermind J, Bülow J: Blood flow in the juvenile hip in relation to changes of the intraarticular pressure. Acta orthop. scand. 1983, 54, (182–187).
Lundsgaard JS, Jensen B, Gronlund J: Fast-responding flow-independent blood gas catheter for oxygen measurement. J. appl. Physiol. 1980, 45, (376–381).
Phelps P, Steele AD, McCarty DJ: Significance of xenon-133 clearance rate from canine and humanjoints. Arth & Rehum. 1972, 15, (360–370).
Svalastoga E, Gronlund J: Experimental osteoarthritis in the rabbit. II. A new method to estimate the oxygen consumption and diffusion capacity in the synovial membrane of the knee. Acta vet. scand. 1985, 26, (326–339).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Svalastoga, E., Kiœr, T. & Grønlund, J. Improved Method to Estimate Oxygen Consumption, Diffusing Capacity and Blood Flow of Synovial Membrane. Acta Vet Scand 30, 113–119 (1989). https://doi.org/10.1186/BF03548046
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1186/BF03548046