Skip to main content
Download PDF
  • Published:

Equine Herpes Virus 1 (EHV-1) in Liver, Spleen, and Lung as Demonstrated by Immunohistology and Electron Microscopy

Acta Veterinaria Scandinavica volume 30, pages 141–146 (1989)Cite this article

Abstract

Ten aborted foals, diagnosed as infected with Equine Herpes Virus 1 (EHV-1) on histopathological criteria, were examined for the presence of EHV-1 using immunohistology as the investigative instrument. The primary reagent was an antiserum specific for viral envelope glycoproteins. Immunohistology localised EHV-1 to areas of liver necrosis and to the cytoplasm of infected Kupffer cells and hepatocytes. Cytoplasmic immunolabelling was also prominent in reticular cells of the red pulp of the spleen and in intact and degenerated bronchiolar epithelium. Cytoplasmic immunolabelling was seen in morphologically unchanged cells and in cells containing intranuclear inclusion bodies. Three aborted foetuses with no histological signs of EHV-1 infection were negative when immunostained for EHV-1.

Detection by electron microscopy of EHV-1 virions confirmed the EHV-1 specificity of the immunolabelling procedure.

References

  • Allen G, Bryans J: Molecular epizootology, pathogenesis and prophylaxis of equine herpesvirus-1 infections. Prog. Vet. Microbiol. Immun. 1986, 2, (78–144).

    CAS  Google Scholar 

  • Arhelger R, Darlington R, Randall C: An electron microscopic study of equine abortion virus infection in hamster liver. Amer. J. Pathol. 1963, 42, (703–707).

    CAS  Google Scholar 

  • Bradford M: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 1976, 72, (248–254).

    Article  CAS  Google Scholar 

  • Bürki F, Bäriswyl K, Lindt S: Nachweis des Virus der Equiner Rhinopneumonitis als Ursache von Stutenaborten. (Demonstration of equine rhino pneumonitis virus as a cause of abortion in mares). Schw. Arch. Tierheilk. 1965, 107, (386–392).

    Google Scholar 

  • Campbell T, Studdert M: Equine herpesvirus type 1. Vet. Bull. 1983, 53, (135–146).

    Google Scholar 

  • Chowdhury S, Kubin G, Ludwig H: Equine herpesvirus type 1 (EHV-1) induced abortions and paralysis in a lipizzaner stud: a contribution to the classification of equine herpesviruses. Arch. Virol. 1986, 90, (273–288).

    Article  CAS  Google Scholar 

  • Darlington R, Moss L: Herpesvirus envelopment. J. Virol. 1968, 2, (48–55).

    Article  CAS  Google Scholar 

  • Darlington R, Moss L: The envelope of herpesvirus. Progr. Med. Virol. 1969, 11, (16–45).

    CAS  Google Scholar 

  • Fong C, Hsiung G: Development of an equine herpesvirus in two cell culture systems: light and electron microscopy. Infect. Immunity. 1972, 6, (865–876).

    Article  CAS  Google Scholar 

  • Gimeno E, Nosetto E, Martin A, Galosi C, Ando Y, Etcheverrigaray M: Demonstration of equine herpes virus 1 (EHV-1) in histological sections and tissue cultures by the peroxidaseantiperoxidase technique. J. Vet. Med. 1987, 34, (740–742).

    Article  CAS  Google Scholar 

  • Howard CR, Sundquist B, Allan J, Brown SE, Chen S-H, Morein B: Preparation and properties of immune-stimulating complexes containing hepatitis B virus surface antigen. J. gen. Virol. 1987, 68, (2281–2289).

    Article  CAS  Google Scholar 

  • Jeleff W: Beitrag zur fetalen Histopathologie des Virusabortes der Stute mit besonderer Berücksichtigung der Differential Diagnose. (Contribution to the fetal histopathology of equine virus abortion, with special attention to the differential diagnosis). Arch. Exp. Vet. Med. 1957, 11, (906–920).

    Google Scholar 

  • Kyhse-Andersen J: Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J. Biochem. Biophys. Methods. 1984, 10, (203–209).

    Article  CAS  Google Scholar 

  • Laemmli U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680.

    Article  CAS  Google Scholar 

  • Morein B, Sundquist B, Höglund S, Dalsgaard K, Osterhaus A: ISCOM. A novel structure for antigenic presentation of membrane proteins from enveloped viruses. Nature. 1984, 308, (457–460).

    Article  CAS  Google Scholar 

  • Nii S, Morgan C, Rose H: Electron microscopy of herpes simplex virus. J. Virol. 1968, 2, (517–536).

    Article  CAS  Google Scholar 

  • O’Neill F, Issel C, Henk W: Electron microscopy of equine respiratory viruses in organ cultures of equine fetal respiratory tract epithelium. Amer. J. Vet. Res. 1984, 45, (1953–1960).

    Google Scholar 

  • Prickett M: The pathology of disease caused by equine herpesvirus 1. Proc. 2nd Int. Conf. Equine Infectious Diseases 1970, p. 24–33.

  • Turtinen I: Studies on the antigenic and genetic variation between the two subtypes of equine herpesvirus-1. PhD. diss., University of Kentucky, 1983.

  • Westerfield C, Dimock W: The pathology of equine virus abortion. J. Amer. vet. med. Ass. 1946, 109, (101–111).

    CAS  PubMed  Google Scholar 

  • Wong F, Spearman J, Smolenski M, Loewen P: Equine parvovirus: initial isolation and partial characterization. Can. J. comp. Med. 1985, 49, (50–54).

    CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

The authors wish to thank Miss Cajsa Magnusson, Mrs. Lena Ekman and Mr. Bengt Ekberg for technical assistance and Mrs. Ulla Malmström for preparing the manuscript. Prof. Claes Rehbinder and Ass. Prof. Berndt Klingebom are gratefully acknowledged for critically evaluating the results.

Author information

Authors and Affiliations

  1. Laboratory of Pathology and the Division of Vaccine Research, National Veterinary Institute, P. O. Box 7073, S-750 07, Uppsala, Sweden

    Lars Jönsson, Johan Beck-Friis, Lena H. M. Renström, Tapio Nikkilä, Per Thebo & Bo Sundquist

Authors
  1. Lars Jönsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Johan Beck-Friis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lena H. M. Renström
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tapio Nikkilä
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Per Thebo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Bo Sundquist
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jönsson, L., Beck-Friis, J., Renström, L.H.M. et al. Equine Herpes Virus 1 (EHV-1) in Liver, Spleen, and Lung as Demonstrated by Immunohistology and Electron Microscopy. Acta Vet Scand 30, 141–146 (1989). https://doi.org/10.1186/BF03548050

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03548050

Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147